JOURNAL OF COASTAL DEVELOPMENT
Vol 13, No 2 (2010): Volume 13, number 2, Year 2010

PROBIOTIC EFFECT OF Lactobacillus isolates AGAINST BACTERIAL PATHOGENS IN FRESH WATER FISH

D Dhanasekaran (Unknown)
Subhasish Saha (Unknown)
N Thajuddin (Unknown)
M Rajalakshmi (Unknown)
A Panneerselvam (Unknown)



Article Info

Publish Date
19 May 2011

Abstract

A total of 59 Lactobacillus isolates were isolated from 5 different fresh water fish such as Cat fish ( Clarias orientalis)  , Hari   fish (Anguilla sp), Rohu fish ( Labeo rohita),  Jillabe  fish (Oreochromis sp) and Gende  fish    ( Punitus carnaticus). Among the 59 isolates only 4 Lactobacillus isolates were selected for further study. Based on morphological and biochemical characteristics, the isolates were identified as Lactobacillus sp. The pathogen were isolated from infected cat fishes, characterized and identified as Vibrio parahaemolyticus, Aeromonas sp and Aeromonas salmonicida. The Lactobacillus isolates were screened for antagonistic activity against Aeromonas, Vibrio sp. by agar diffusion assay. Among the 4 isolates, Lactobacilli RLD2 showed significant antagonistic activity against Aeromonas and Vibrio sp alone. and was further evaluated by standard plate count assay for the viability of pathogen. The isolate was multiplied and the fish feed was supplement with Lactobacillus isolates. The results reveal that the size, weight of the fish was statically increased in comparison to that of control fish. The present study concluded that the Lactobacillus isolates could be used as probiotic bacteria in aquaculture, to manage aeromonasis.  

Copyrights © 2010






Journal Info

Abbrev

coastdev

Publisher

Subject

Education

Description

The Journal of Coastal Development (ISSN 1410-5217) is dedicated to all aspects of the increasingly important fields of coastal and marine development, including but not limited to biological, chemical, cultural, economic, social, medical, and physical development. The journal is jointly published ...