This research was aimed to know the effect of A3-51 probiotic supplementation on food to the total number of bacteria in kidney and mortality of nila fish (Oreochromis niloticus). This research was carried out in two steps, the first was to know the highest immunity level from fish indicated by the increasing number of the macrophage and its phagocytic activity. Meanwhile, the second was to know the immunity level of fish which injected by Aeromonas hydrophila and atypical Aeromonas salmonicida. The experimental method used was Completely Randomized Design in 3 replicates. The result from the first step showed that the highest non specific immune system was found in 21 days treatment. The second step showed that the highest total number of bacteria in kidney and mortality level were 20.23 x 10 cells/g and 46.67% respectively, both was found in B2 treatment. Key words: probiotic, macrophage, total number of bacteria, mortality
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