Seleksi Primer LCO – HCO, Primer bird-f1 – HCO Dan Primer bch – bcl Untuk Amplifikasi Gen COI DNA Mitokondria Itik Magelang (Anas javanica)

Setiyawan, Sonny Abdi
Budiharjo, Anto
Kusumaningrum, Hermin Pancasakti

Bioma : Berkala Ilmiah Biologi
Vol. 16, No.2, Tahun 2014

Magelang duck is a wild type of local duck from Indonesia. The advantagesof Magelangduckcompare tootherlocalduck from Indonesiaareabilityto livein the highlandsandlowlands and high production of egg and meat. Geneticcharacterization of Magelangduck still not available until now.The aim of the research is selectprimers forampliflying COIgeneof mitochondrialDNAof MagelangduckusingLCO-HCO, bird-f1 -HCO, andbcl-bch primers.The research methodwas DNAisolationfrom Magelangduck. Followed by, selection of primer in silicoto find homologywithin COIsequenceusing ClustalX, Genedoc, and FastPCR programs. Amplification of COIgenewas performedusing PCRwith all primerpairs. Result showed partial homology with all primer in COI sequence. TheamplificationusingtheLCO-HCO primer produced  primerdimer.Primerbirdf1-HCOand bch-bcl primers showed no amplification.   Key words: Magelang duck, COI gene, mitochondrial DNA, primer