Bioma : Berkala Ilmiah Biologi
Vol. 15, No.1, Tahun 2013

Optimasi Isolasi DNA Cabai (Capsicum annuum L.) Berdasar Perbedaan Kualitas dan Kuantitas Daun serta Teknik Penggerusan

Ferniah, Rejeki Siti (Unknown)
Pujiyanto, Sri (Unknown)

Article Info

Publish Date
14 Jun 2013

Abstract

Complete genome of chili has not been reported.The first step to study the genome is DNA isolation, so it is necessary to optimize the protocol to get an optimum DNA. This research aimed to optimize chili DNA isolation by variate the quantity and quality of chili leaves as row material and variate the grinding technique. DNA isolation was done using commercial kit without liquid nitrogen, and analyzed by agarose gel electrophoresis. The results showed that frozen chili leaf yields more DNA than fresh leaf, 0,1 g of leaf got optimum DNA, and grinding in mortar was better than in microtube.   Key words: DNA,  isolation, Capsicum annuum

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Journal Info
Bioma : Berkala Ilmiah Biologi
Website

Abbrev

bioma

Publisher

Universitas Diponegoro

Subject

Agriculture, Biological Sciences & Forestry Education

Description

BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata ...