<![CDATA[An increase of population growth and energy demand has lead to a foster fuel consumption, therefore an alternative fuel is necessarily needed for a solution to solve the problem. Currently, one of the most popular solution offered is biodiesel. The development of biodiesel as renewable energy is done by using microalgae such as Chlorella sp. with 28-32% lipid content as its raw material. The objective of this research is to find out the effect of various concentration, biomass based on growth time, and lipid content based on growth time result by comparing it to the addition of synthetic nutrients and whey (5%, 10%, 15%, 20%, and 25% Total Volume). The optimal growth on cultivation of Chlorella sp. with the addition of synthetic nutrients occurs in day 9 with 0,487 Optical Density (OD), 2,0818 grams/litre of dry biomass, and 0,493 grams/liter of lipid. While for Chlorella sp. cultivation with the addition of tofu waste water (whey) has its optimum condition for Optical Dansity occurs in day 9 of 20%V whey addition which is 0,486. Also observed in day 9, the optimum condition for dry biomass and lipid of 25%V whey addition is 4,2125 grams/litre and 1,081 grams/litre. Chlorella sp. cultivation using 25%V whey addition produced better result comparing to cultivation by using synthetic nutrient.The maximum COD reduction occurs in 15%V whey addition with 77,01% reduction efficiency, while on the other hand, with the addition of whey in its optimum variable (25%V) resulting a decrease of efficiency by 64,28%. Based on tofu industrial center located in Jomblang, with 7 quintals per day of soybean as raw material, it can produce lipid for biodiesel feedstock amounted up to 0,188 kg per day.]]>
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