JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Vol. 6 No. 2 (2019): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA

The Effect of Vitamin C Addition on Epigallocatechin Gallate (EGCG) Stability in Green Tea Solution

Alief Putriana Rahman (Master of Pharmaceutical Science, Faculty of Pharmacy, Universitas Airlangga, Surabaya)
Djoko Agus Purwanto (Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya)
Isnaeni Isnaeni (Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya)



Article Info

Publish Date
04 Mar 2020

Abstract

Background: Epigallocatechin gallate (EGCG) is the most abundant green tea catechin with a powerful antioxidant effect to prevent cancer cells. EGCG in green tea solution is highly susceptible to degradation, this it is urgent to  increase the stability of EGCG by vitamin C addition. Vitamin C can regenerate radical EGCG to be normal EGCG. Objective: This research aim was to determine percent of decreased level of EGCG before and after vitamin C addition. Methods: This paper was focused on enhancing the stability of EGCG by 1 mg (GTVC1), 1.5 mg (GTVC2), 2 mg (GTVC3), 2.5 mg (GTVC4) and 3 mg (GTVC5) of vitamin C addition to 10 g/L of green tea solution concentration. Evaluations of EGCG were conducted at 0 days, 1 day, 2 days, 3 days, and 4 days of storage time. EGCG was analyzed using thin layer chromatography (TLC) densitometry methods. The stability of EGCG was determined by % of decreased EGCG. Results: Percent of loss of EGCG in GT, GTVC1, GTVC2, GTVC3, GTVC4 and GTVC5 after 4 days storage were 19.93%, 10.89%, 21.08%, 18.18%, 28.56%, and 9.76%, respectively. Conclusion: This study showed that in 4 days storage, the decreased level of EGCG in green tea solution with 3 mg of vitamin C addition (GTVC5) was 9.76% which was smaller than green tea solution without vitamin C addition (GT) which EGCG decreasing 19.93%.

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Journal Info

Abbrev

JFIKI

Publisher

Subject

Chemistry Education Health Professions Medicine & Pharmacology Other

Description

Jurnal ini adalah jurnal peer-review nasional, yang diterbitkan dua kali dalam membahas tentang topik-topik hasil penelitian di bidang pelayanan dan praktik kefarmasian, konsultasi masyarakat, teknologi kefarmasian serta disiplin ilmu kesehatan yang terkait dengan erat. Jurnal ini memfokuskan pada ...