Inhibition of Lipid Peroxidation Induced by Ultraviolet Radiation by Crude Phlorotannis Isolated from Brown Algae Sargassum hystrix v. buxifolium C. Agardh

Agnes Nora Iska Harnita (Faculty of Pharmacy, Sanata Dharma University, Yogyakarta)
Ign. Edi Santosa (Dept of Physics Education, Sanata Dharma University, Yogyakarta)
Sudibyo Martono (Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta)
Sudarsono Sudarsono (Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta)
Sitarina Widyarini (Faculty of Veterinary Science, Universitas Gadjah Mada, Yogyakarta)
Frans J.M. Harren (Life Science Trace Gas Facility, Molecular and Laser Physics, Radboud University, Nijmegen)

Indonesian Journal of Chemistry
Vol 13, No 1 (2013)

This study examines the antioxidant activity of crude phlorotannins from the brown algae Sargassum hystrix v. buxifolium (Chauvin) J. Agardh, through the inhibition of a lipid peroxidation reaction that is induced by the UV radiation. The antioxidant activity during the UV exposure was investigated using the laser-based photoacoustic method for the detection of the ethylene as indicator for lipid peroxidation. This involves an experiment that isolated crude phlorotannins from the ethyl acetate fraction of the Sargassum hystrix methanol extract, hereafter referred to as PFSH. It results in the antioxidant activity as a potent lipid peroxidation inhibitor. Statistically, such antioxidant activity is not significantly different than the commercial antioxidant, which is vitamin C (p > 0.05). The amount of the total phlorotannins, using the Folin-Ciocalteu method, was measured to be approximately 0.13% w/w. In addition, it is found that PFSH contains phlorotannins with low molecular weight (MW) (