In this research, we investigated the effects of supplementation of rice bran fermented with Effective Microorganism (EM4) on the biomass of tubifex worms cultured in the recirculation system. A Completely randomized design was made, consisting of 4 treatments and 3 replications. The experimental animals were treated with different types of feed, i.e. (A) unfermented rice bran, three times a day; (B) fermented rice bran, three times a day; (C) fermented rice bran, 5 times a day; (D) fermented rice bran, 7 times a day. They were reared in a wood box (100 × 50 × 15 cm3), while a mixture of fermented rice bran and mud was applied as their growth medium. Tubifex worms were grown in the medium at a density of 100 g/m2, and supplemented with fermented rice bran at a dose of 500 g/box, with various frequency levels of 3, 5 and 7 times a day. Biomass of the worm and water quality was observed. As a result, we found that the addition of fermented rice bran at a rate of 5 times a day (treatment C) exhibited the greatest biomass growth, reaching up to 104,33 g/m2.
                        
                        
                        
                        
                            
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