This study aims determine the influence of soaking water exchange time and commercial fungal doses to rubber seed tempeh. This research used randomize block design (RBD) 2 factorial combination were (1) soaking water exchange time 6, 9 and 12 hour, and (2) combination fungal doses 0,01, 0,1 dan 1 %(b/b.) Observation parameters to be measured content of, ash, protein, fat, carbohydrate, and HCN. Result were analyzed with Anova and BNJ 5% level test. The results of the study showed a combination of soaking water exchange time 12 hour and commercial fungal doses 1% the best treatment. Chemical characteristic of rubber seed werecontent water 54.2%, ash 1.01%, protein 27.51%, fat 16.31%, carbohydrate 3.75% and HCN 4.64 mg/L.
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