Agrosains: Jurnal Penelitian Agronomi
Vol 22, No 1 (2020): Agrosains : Jurnal Penelitian Agronomi

Induksi Kalus Gaharu dengan NAA dan BAP Secara In Vitro

Amalina Wahyuni (Departement of Agrotechnology, Faculty of Agriculture, Universitas Andalas, Padang, Indonesia)
Benni Satria (Departement of Agrotechnology, Faculty of Agriculture, Universitas Andalas, Padang, Indonesia)
Aprizal Zainal (Departement of Agrotechnology, Faculty of Agriculture, Universitas Andalas, Padang, Indonesia)



Article Info

Publish Date
01 Apr 2020

Abstract

Agarwood has a high economic value because they have many benefits that cause agarwood to be hunted to meet the increasing market demand and its presence in nature is decreasing due to unwise conventional retrieval systems.The research objectives were to study the effect and determine the best concentration of NAA and BAP in callus induction of agarwood (Aquilaria malaccensis Lamk.). This research was conducted in the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University from October 2018 until January 2019. The leaves of Agarwood were used as explants. The method used was completely randomized design (CRD) with different combinations of NAA and BAP (1.5 + 0.5 ppm, 3.0 + 0.5 ppm, 1.5 + 1.0 ppm, and 3.0 + 1.0 ppm). Data were analyzed using the F test and continued with Duncan’s Multiple Range Test at a level of 5%. The results showed that there was the effects of combination of growth regulator substances NAA and BAP on callus induction of agarwood. The concentration of NAA 3.0 ppm + BAP 0.5 ppm was the best in inducing callus with the percentage of explants life and explants form callus with 100%. The color of callus obtained varies, yellow, brownish yellow and brown. All structures callus gained in this research was compact.  Yellow callus and compact structure could be utilized for organogenesis.

Copyrights © 2020






Journal Info

Abbrev

agrosains

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Agrosains: Jurnal Penelitian Agronomi publishes articles related to agriculture such as plant physiology, crop production, physiology and seed production, soil science, plant breeding, plant protection, agro-climatology, agroecology, and plant biotechnology. ...