Jurnal Ilmu dan Teknologi Kelautan Tropis
Vol. 3 No. 2 (2011): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis

SCREENING OF INDONESIAN ORIGINAL BACTERIA VIBRIO SP AS A CAUSE OF SHRIMP DISEASES BASED ON 16S RIBOSOMAL DNA-TECHNIQUE

Feliatra Felix (Unknown)
Titania T Nugroho (Unknown)
Sila Silalahi (Unknown)
Yuslina Octavia (Unknown)



Article Info

Publish Date
31 Dec 2011

Abstract

Shrimp disease caused by Vibrio sp is one of the main limiting factors in the increasing production in shrimp farming. This disease may kill the shrimp and cause high loss in shrimp culture in South East and East Asia. Samples of 10 individuals of a ten months cultured giant tiger prawn as well as 1 liter pond water and sea waters were collected from shrimp pond in Bengkalis Island, Sumatra. Samples of shrimps were also collected from Jepara shrimp pond in Central Java and they were selected by looking at their behavior and unhealthy physical characteristics. Amplification, rDNA 16s sequencing and bioinformatics analysis to identify Vibrio species were conducted in Biotech Center, BPPT Serpong Banten. The results of DNA sequencing of each bacteria isolate were compared to DNA sequence from international DNA bank database. Tracing were made by BLAST (Basic Local Alignment Search Tool) system accessed through the internet at http://www./ncbi.nlm.nih.gov/blast. The result of this study found seven strains of Vibrio sp bacteria where five of which (V. alginolyticus, V. parahaemolyticus, V. harveyi, V. shilonii and V. vulnificus) were already registered in the world gen bank with homolog level above 97%. Meanwhile, another two strain found in this study were not found in the list of world gen bank and therefore are considered as native Vibrio sp bacteria from Indonesia.Keywords: Vibrio sp, bacteria, shrimp, aquaculture, fish disease

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Journal Info

Abbrev

jurnalikt

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Library & Information Science

Description

Aims and Scope Journal of Tropical Marine Science and Technology (Jurnal Ilmu dan Teknologi Kelautan Tropis) is a scientific journal in the field of tropical marine science and technology. We have a aims and scope to focus in publishing a good quality scientific articles for dissemination of ...