DIVERSITY OF ACTINOMYCETES FROM EKA KARYA BOTANICAL GARDEN, BALI

Shanti Ratnakomala (Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong 16911)
Puspita Lisdiyanti (Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong 16911)
Nita Rosalinda Prayitno (Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong 16911)
Evi Triana (Research Center for Biology, Indonesian Institute of Sciences, Cibinong 16911)
Yulin Lestari (Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, Bogor 16680)
Ratih Dwi Hastuti (Soil Research Institute, Bogor 16002)
Yantyati Widyastuti (Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong 16911)
Misa Otoguro (INITE Biological Resource Center (NBRC), 2-5-8 Kasuzakamatari, Kisarazu, Chiba)
Katsuhiko Ando (NITE Biologycal Resource Center (NBRC))
Endang Sukara (Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong 16911)

BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Vol. 23 No. 1 (2016): BIOTROPIA Vol. 23 No. 1 June 2016

A total of 29 actinomycetes strains were isolated and identified by full sequence analysis of the 16S rRNA gene. Samples were collected from Bali Island, Indonesia, including 18 soil and 20 leaf litter samples from Eka Karya Botanical Garden. Two isolation methods were used in this study: SDS-extract (SY) and Rehydration-Centrifugation (RC). Based on 16S rRNA gene analysis, the isolated actinomycetes were grouped into two genera. Molecular analysis of 16S rRNA gene similarities showed that the actinomycetes isolated from Eka Karya Botanical Garden were of diverse origin. Analysis of soil samples resulted in 144 isolates belonging to 24 genera and more than 87 species, with Streptomyces being the dominant genus. This was followed by Actinoplanes (25 isolates, 17%). From leaf litter samples, 85 isolates were obtained, belonging to 9 genera and more than 41 species. The most dominant genus was Catenuloplanes (42 isolates, 49%), followed by Actinoplanes (16 isolates, 19%).