Journal of Health Sciences and Medicine
Vol 1 No 2 (2017): JHSM (September 2017)

Shiga Like Toxin 1 (STX-1) Detection From Escherichia coli O157:H7 Local Isolates

I W. Suardana (Udayana University Bali-Indonesia)
K.J. P. Pinatih (Udayana University Bali-Indonesia)
D. A. Widiasih (Gadjah Mada University Yogyakarta-Indonesia)



Article Info

Publish Date
25 Jan 2018

Abstract

Shiga-like toxin (Stx) otherwise known as verotoxin and verocytotoxin is a toxin produced by some strains of Escherichia coli particularly by strain O157: H7. This toxin is an AB5 toxin type which is known to have similarities with the toxins produced by Shigella dysenteriae . Stx from E. coli O157: H7 can be distinguished into stx 1 and stx 2. Stx1is usually associated with most outbreaks and detrimental sporadic cases of illness in humans. In this research, we observed the titer of Shiga toxin (Stx 1 / VT1) from local isolates isolated from cattle and human feces by using vero toxin Escherichia coli -reverse passive latex agglutination test (VTEC-RPLA) method. The results showed local isolates KL-48 (2) of human origin, SM-25 (1) of cattle feces origin and DS 21 (4) of beef origin positively produced VT1 2 units of titer, meanwhile the isolate SM 7 (1) was negative. Titer of toxin Stx1 produced from local isolates was known to be lower when compared to the control isolate ATCC 43894 with 8 units of titer.

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Journal Info

Abbrev

JHSM

Publisher

Subject

Medicine & Pharmacology

Description

Journal of Health Sciences and Medicine is an Indexed peer-reviewed open access journal which publishes original scientific articles, reviews and short communications that are not under consideration for publication elsewhere. The journal publishes papers based on original research that are judged ...