Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

Journal of Health Sciences and Medicine

JHSM
Website

Published by Universitas Udayana

ISSN : 25497561 EISSN : 26220555 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Journal of Health Sciences and Medicine is an Indexed peer-reviewed open access journal which publishes original scientific articles, reviews and short communications that are not under consideration for publication elsewhere. The journal publishes papers based on original research that are judged by critical reviews, to make a substantial contribution in the field. It aims at rapid publication of high quality research results while maintaining rigorous review process. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers are published approximately one month after acceptance.

Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)

Articles 25 Documents

1 2 3

Occurrence of Hypoglycemia, Hypokalemia and Hyperkalemia in Diabetic Hypertensive Patients Using Insulin and Diuretics Sarasmita M A; Setyawan E. I.; Hendra Jaya A.
Journal of Health Sciences and Medicine Vol 1 No 2 (2017): JHSM (September 2017)
Publisher : Institute for Research and Community Services Udayana University

Diabetes Mellitus (DM) is a metabolic disease with characteristics of hyperglycemia that occurs due to insulin secretion abnormalities, insulin work or both. DM can cause macrovascular complications such as hypertension. Administration of therapy may lead to hypoglycemic and hypokalemia that may increase the risk of impaired brain and heart. Objective: The purpose of this study to analyze the incidence of hypoglycemia and hypokalemia side effects in patients with diabetic hypertension using antidiabetes and antihypertensives. Method: The study was conducted retrospectively with retrospective patient medical records from January to December 2016. Blood and potassium blood glucose levels were measured as long as the patient was treated. Random and potassium blood glucose levels prior to therapy were compared with after therapy using paired t-test. Result : From the results of the study found 27 patients suffering from diabetic hypertension and get therapy. The value of changes in the patient's random blood glucose level averaged 280.89 mg/dl to 189.15 mg/dl, which met the target of antidiabetic drug therapy, and no patients had hypoglycemia. From 27 patients, as many as 7 people did not get antihypertensive therapy. Three patients had hyperkalemia (serum potassium level > 5 mEq/L). The value of changes in blood potassium levels of patients averaged 4.74 mEq/L to 4.46 mEq/L, where no patients had hypokalemia. Conclusion : the occurrence of hypoglycemia and hyperkalemia were suspected due to side effect of insulin and antihypertensive drugs.

ISOLATION AND PHYTOCHEMICAL TEST OF ANTICANCER ISOLATE OF SPONGE Hyrtios erecta I Made Dira Swantara; Wiwik Susanah Rita; Rr Anisa Hernindy
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

AbstractThis research aims to isolate and phytochemically test of the toxic isolate from ethanol extract of the sponge Hyrtios erecta taken from the waters of Pari Island beach, Thousand Islands (Jakarta). Extraction of the sponges was carried out by 70% ethanol at room temperature. Partition and purification of the compounds were done by column chromatography with the stationary phase of silica gel and the mobile phase of n-hexane-chloroform (2:8). Toxicity screening test was done based on Bhrine Shrimp Lethality Test (BSLT). The compounds of the active isolate were performed by phytochemical test. Based on the results, it was found that the toxic isolate of Hyrtios erecta sponges has anticancer activity with IC50 of 30,497 ppm. The anticancer isolate contained alkaloid, steroid, and polyphenol compounds

SCREENING POTENTIAL ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF PROTEIN HYDROLYSATES DERIVED FROM GERMINATED LABLAB BEAN, PIGEON PEA AND KIDNEY BEAN Ketut Ratnayani; Indriani Wisnu Susanto Panjaitan; Ni Made Puspawati
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

Abstract Protein hydrolysate contains a mixture of various lengths of short peptides chain and free amino acids that may excert biological activities. This research aims to screen potential antioxidant and antibacterial activities of protein hydrolysate produced from three kinds of germinated beans i.e. lablab bean (Lablab purpureus), pigeon pea (Cajanus cajan (L.) Millsp) and kidney bean (Phaseolus vulgaris) through enzymatic hydrolysis process. The steps of research included germination process of the beans prior to total protein isolation, enzymatic hydrolysis of total protein isolates using pancreatin enzyme, evaluation of in vitro antioxidant activity of the hydrolysates protein using DPPH (1,1-diphenyl-2-picryl hydrazyl) method, and antibaterial activity testing towards Eschericia coli and Staphyllococcus aureus bacteria. The results revealed that pancreatine enzyme was able to hydrolyse germinated protein of lablab bean, pigeon pea and kidney bean at the experiment condition applied with degree of hydrolysis 34.12%, 27.44%, and 30,93% respectively. It was also found that protein hydrolysates of lablab bean, pigeon pea, and kidney bean demonstrated antioxidant activity which percentage radical DPPH scavenging activity of 84.02%, 68.97% and 67.89 %. On the other hand, all of those protein hydrolysates did not show any antibacterial activity towards Eschericia coli and Staphyllococcus aureus bacteria.

Validation of Method for Determining Alpha- Mangostin Level of Ethyl Acetate Extract of Mangosteen Rind Using TLCSpectrodensitometry N. P. A. D. Wijayanti; L.P.M.K. Dewi; K.W. Astuti
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

Abstract Objective: Mangosteen rind (Garcinia mangostana L.) contains secondary metabolites, namely alpha-mangostin which has antioxidant activity, as well as antibacterial and anti-aging properties. In order to obtain a maximum amount of alpha-mangostin compounds, the maceration method using ethyl acetate was used. To ensure the effectiveness of the mangosteen rind extraction process, all of the processes and methods in preparing the extract should be properly controlled, particularly the analytical method used in determining the alpha-mangostin level of the extract. The method used should be able to determine the alphamangosteen level accurately. This study aimed to test the validation of the analytical method used. Method: The parameters for the validation of the analytical method tested in this study include accuracy, precision, range and linearity, limit of detection (LOD), limit of quantitation (LOQ) and specificity. In this study, the level of alpha-mangostin in the extract was determined using TLC-Spectrodensitometry with the stationary phase of the silica gel plate GF 254 and the mobile phase of chloroform and methanol (10:0.1 v/v). Result: The results of the study showed that this method has met the acceptance criteria for validation with the accuracy value in the range of percent recovery, from 93.85 % to 111.16%; precision with KV <2%; specification with spectrum correlation >0.99; linearity with r =0,99372; limit of detection (LOD) of 5.33 ng and limit of quantitation (LOQ) of 11.43 ng.

CHARACTERISTICS OF AMBLIOPIA IN ANISOMETROPIC PATIENTS AT EYE POLYLINIC SANGLAH GENERAL HOSPITAL DENPASAR Ni Putu Pradita Dhriti Indriyani Sukadan
Journal of Health Sciences and Medicine Vol 2 No 1 (2018): JHSM (February 2018)
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.25 KB)

The purpose of this cross sectional study was to determine the characteristics of amblyopia in anisometropic patients at Eye Polyclinic Sanglah General Hospital Denpasar.The data hat obtained are secondary data derived from medical records of refractive error in anisometropic patients on April 2016-August 2017 period, and involved 62 patients as research subjects based on consecutive sampling.The results of this study showed that from 62 anisometropic patients, there were 56 patients (90%) affected amblyopia. Based on the sociodemographic aspect, the highest number of patients with amblyopia in the children age group were 24 peoples (43%), females more than males were 37 peoples (66%), the majority of patients living in Denpasar were 28 peoples (50%), and the students were the most type of occupationin amblyopic patients is 35 peoples (63%). Patients with severe anisometropia were 24 peoples (43%), moderate amblyopia at 48 eyes (43%), and affected on both eyes (ocular dextra sinistra) about 35 peoples (63%). The type of refractive error was found to be at most is astigmatism about 74 eyes (66%), and the use of spectacles in 51 people (91%).Based on this research, it can be concluded that subject who were affected amblyopia in anisometropic patients most at age children, female, living in Denpasar, and as students. Severe anisometropia, moderate amblyopia, affected on both eyes, astigmatism, and the uses of spectacles were found to be the most of the subject.

The Determinants of Ethical Principle Implementation in Nursing Ni Putu Emy Darmayanti; Hanny Handiyani; Kuntarti Kuntarti
Journal of Health Sciences and Medicine Vol 1 No 2 (2017): JHSM (September 2017)
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.882 KB)

The determinant of nursing ethics practice is not identified yet. This problem affects the ethical behavior of nurses in performing nursing care. According to that, it was influencing satisfaction and patient well-being. This study aims to identify the determinants of the ethical principles application in nursing. Descriptive, correlation, cross-sectional design was applied, through simple random sampling to 120 nurses. Data collecting used questionnaires, with univariate, bivariate, and multivariate analyzes. Determinants of nursing ethic application principle were job characteristics, nurses knowledge related to nursing ethics, supervision by supervisor, relationship with colleagues, awards, the role of head nurse, and tenure (p = <0,001-0,049). The most dominant variable was job characteristics (p <0,001). These factors need an attention by hospital manager to keep evaluating the other factors in order to improve the application of ethical principles in nursing.

CAPSULAR GENOTYPING OF Klebsiella pneumoniae ISOLATED FROM CLINICAL SAMPLES AT SANGLAH GENERAL HOSPITAL IN 2013 I Made Sutha Saskara; Ni Nengah Dwi Fatmawati; Ni Nyoman Sri Budayanti; Wahyu Hidayati
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

Klebsiella pneumoniae, one of hospital associated infections agents, become more prevalence in worldwide. It is an opportunistic pathogen that can cause wide spectrum of diseases such as pneumonia and sepsis. Capability of this bacterium in causing diseases is influenced by its virulence factors. Capsule (K) is considered as the major virulence factor responsible in avoiding first defense mechanism of host cells. Almost no study about molecular characterization of capsule is reported in Bali; therefore, this study is aimed to determine the capsular genotype of K. pneumoniae isolated from clinical specimens at Sanglah General Hospital. Samples were collected between January until Desember 2013 in Sanglah General Hospital at Clinical Microbiology Laboratory. Total of 56 samples were examined taken from blood, urine, pus, sputum, and other sources. All K. pneumoniae DNA were then subjected to PCR using specific primer pairs against K1, K2 and K5. The results showed that from 56 K. pneumoniae clinical isolates only 12 (21.4%) were PCR positive, and all (100%) of them were positive for K2 capsule gene. Ten (83.3) of them were from blood, 1 (8.3%) from sputum, and 1 (8.3%) from other specimens. Finding of K2 capsule gene in most K. pneumoniae clinical isolates at Clinical Microbiology Laboratory in Sanglah General Hospital might figure out the relationship of capsular type with severity of diseases. However, further study of capsule in Bali with higher isolates number will help in understanding of pathogenicity of K2 capsule in order to treat the infections itself.

IN SILICO PRIMER DESIGN AND ANNEALING TEMPERATURE OPTIMIZATION TO AMPLIFY THE FRAGMENT OF gyrB GENE Mycobacterium tuberculosis ISOLATE P010 USING POLYMERASE CHAIN REACTION Putu Irma Handayani
Journal of Health Sciences and Medicine Vol 2 No 1 (2018): JHSM (February 2018)
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.178 KB)

One of the factors causing XDR-TB is due to mutations in the Mycobacterium tuberculosis gene, one of them is in the gyrB gene. Amplification of gyrB gene fragments from Mycobacterium tuberculosis DNA using Polymerase Chain Reaction (PCR) method. The amplification process by the PCR method requires a pair of primers (forward and reverse) to limit the area to be amplified. The current study aims to obtain the best primer pair generated by in silico design using Clone Manager Suite 6 program while simultaneously optimizing the annealing temperature to amplify the fragment of gyrB Mycobacterium tuberculosis. The template used in designing the primer is the sequence of gyrB Mycobacterium tuberculosis H37Rv isolate obtained from NCBI database of genbank code AL123456.3. The current study obtained a pair of primer which respectively had 19 oligonucleotide length and the best annealing temperature of 56ºC. The primer is be able to do in silico amplification of the fragment of gyrB Mycobacterium tuberculosis gene isolate P010 in the nucleotide area range from 1271-1755 bp with 485 bp fragment length.

Writing Guidance and Cover Belakang Sagung Chandra Yowani
Journal of Health Sciences and Medicine Vol 1 No 2 (2017): JHSM (September 2017)
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1811.001 KB)

Shiga Like Toxin 1 (STX-1) Detection From Escherichia coli O157:H7 Local Isolates I W. Suardana; K.J. P. Pinatih; D. A. Widiasih
Journal of Health Sciences and Medicine Vol 1 No 2 (2017): JHSM (September 2017)
Publisher : Institute for Research and Community Services Udayana University

Shiga-like toxin (Stx) otherwise known as verotoxin and verocytotoxin is a toxin produced by some strains of Escherichia coli particularly by strain O157: H7. This toxin is an AB5 toxin type which is known to have similarities with the toxins produced by Shigella dysenteriae . Stx from E. coli O157: H7 can be distinguished into stx 1 and stx 2. Stx1is usually associated with most outbreaks and detrimental sporadic cases of illness in humans. In this research, we observed the titer of Shiga toxin (Stx 1 / VT1) from local isolates isolated from cattle and human feces by using vero toxin Escherichia coli -reverse passive latex agglutination test (VTEC-RPLA) method. The results showed local isolates KL-48 (2) of human origin, SM-25 (1) of cattle feces origin and DS 21 (4) of beef origin positively produced VT1 2 units of titer, meanwhile the isolate SM 7 (1) was negative. Titer of toxin Stx1 produced from local isolates was known to be lower when compared to the control isolate ATCC 43894 with 8 units of titer.

Page 1 of 3 | Total Record : 25

1 2 3

Filter by Year

2017 2018

Filter By Issues

All Issue Vol 2 No 1 (2018): JHSM (February 2018) Vol 1 No 2 (2017): JHSM (September 2017) Vol 1 No 1 (2017): JHSM (Febuary 2017)

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
Sagung Chandra Yowani

Contact Email
jhsm@unud.ac.id

Phone
-

Journal Mail Official
jhsm@unud.ac.id

Editorial Address
Institute for Research and Community Services Udayana University Gedung LPPM lantai 4 Bukit Jimbaran, Badung, Bali, Indonesia-80361

Location
Kota denpasar,

Bali

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Sagung Chandra Yowani

Contact Email jhsm@unud.ac.id

Phone -

Journal Mail Official jhsm@unud.ac.id

Editorial Address Institute for Research and Community Services Udayana University Gedung LPPM lantai 4 Bukit Jimbaran, Badung, Bali, Indonesia-80361

Location Kota denpasar, Bali INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Sagung Chandra Yowani

Contact Email
jhsm@unud.ac.id

Phone
-

Journal Mail Official
jhsm@unud.ac.id

Editorial Address
Institute for Research and Community Services Udayana University Gedung LPPM lantai 4 Bukit Jimbaran, Badung, Bali, Indonesia-80361

Location
Kota denpasar,

Bali

INDONESIA