BIOTROPIA - The Southeast Asian Journal of Tropical Biology
No. 16 (2001)

IN VITRO PROPAGATION OF ANGIOPTERIS EVECTA USING SPORES

POONAM BHATIA (School of Biological and Environmental Sciences,)
DAMIEN CUPITT (Primary Industries Research Centre, School of Biological and Environmental Sciences, Central Queensland University, Rockhampton, QLD 4702, Australia)
NANJAPPA ASHWATH (Primary Industries Research Centre, School of Biological and Environmental Sciences, Central Queensland University, Rockhampton, QLD 4702, Australia)



Article Info

Publish Date
16 Nov 2011

Abstract

Techniques of establishing Angiopleris evecta plants  in vitro were studied. Soaking of A. evecta spores in water for 24 hours markedly reduced spore contamination. Soaking of the spores in 1 -2 % of sodium hypochlorite for  less  than 5 minutes allowed satisfactory disinfestation without affecting spore viability. Lower concentration of minerals (1/4 MS), presence of charcoal in the medium and exposure of the spores to light were crucial for spore germination and gainetophytc development of A. evecta. Keywords:   Angiopleris evecta I King fern / spore / MS medium / bleach / light / tissue culture / sporophyte / gamctophytc

Copyrights © 2001






Journal Info

Abbrev

biotropia

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

Description

BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and ...