DAMIEN CUPITT
Primary Industries Research Centre, School of Biological and Environmental Sciences, Central Queensland University, Rockhampton, QLD 4702, Australia

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IN VITRO PROPAGATION OF ANGIOPTERIS EVECTA USING SPORES POONAM BHATIA; DAMIEN CUPITT; NANJAPPA ASHWATH
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 16 (2001)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2001.0.16.161

Abstract

Techniques of establishing Angiopleris evecta plants  in vitro were studied. Soaking of A. evecta spores in water for 24 hours markedly reduced spore contamination. Soaking of the spores in 1 -2 % of sodium hypochlorite for  less  than 5 minutes allowed satisfactory disinfestation without affecting spore viability. Lower concentration of minerals (1/4 MS), presence of charcoal in the medium and exposure of the spores to light were crucial for spore germination and gainetophytc development of A. evecta. Keywords:   Angiopleris evecta I King fern / spore / MS medium / bleach / light / tissue culture / sporophyte / gamctophytc