<![CDATA[At these days nanoparticles (NPs) are used as antibacterial due to its have chemical-physical addition to biological effect. A big group of microbial cells adhering to a surface are called biofilm. Exposure to nano particles such as (Ag, Al2O3, Ni) may prevent colonization of new bacteria onto the biofilm. In the present research we have analyze whether the biofilm formation of some isolates of pathogenic bacteria could be influenced by NPs. Also we examined the susceptibility of the isolates to some antibiotics in combination with Ag, Al2O3 and Ni nanoparticles. (50) Strains of isolated K. pneumonia have been examine from patients with urinary tract infection were collected from Education of Baghdad hospitals in Iraq. Serial dilution of Tube method to determination of MIC and MBEC for Nanoparticles against isolates was done. Biofilm formation was evaluated by micro titer plates. Additionally. disc diffusion method was used to assay the various antibiotics and combinations for bactericidal activity against the isolates. The results showed that about 97% of the strains were capable to form of structure biofilms. More than 82% about of strains showed resistance to commonly used antibiotics. Antibiotics and antimicrobials from mixed nanoparticles were higher than compared to nanoparticles alone. Therefore, a synergistic effect was observed between silver - Nickel nanoparticles and silver - aluminum oxide nanoparticles in to inhibiting the growth of K. pneumonia strains. Furthermore, cytotoxicity activity of Ag NPs against Neuro-2a cells was higher than Al2O3 NPs. The results suggest that nanoparticles can be used as antimicrobial agents for therapeutic to treat urinary tract infections caused by K. pneumoniae.]]>
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