This study was conducted in the Al Batool Teaching Hospital in Ba’aqubah in Diyala city, from August 2019to December 2019. In children under 10 years patients suffering from diarrhea with different gastrointestinalcomplaints, Stool samples were collected from each patients use for microscopic examination and Cultureand sensitivity test. Diarrheal diseases are major problem of developing countries. Though precise data onchildhood mortality associated with diarrheal diseases in Iraq is not available, it has been estimated thatapproximately 25% of child death are associated with diarrheal disease, particularly acute diarrhea. Thepurpose of this study was to detect causative agent of acute diarrhea in children less than 10 years of age.With the study of sensitivity to certain antibiotics. Bacterial pathogen infected cases 30 (60%) male and20(40%) female, among the total enrolled cases the Escherichia coli was 30(60%), Klebsiella species was17(34%) and kluyvera ascorbate species was 3 (6%).also the study detected that bacterial infection wasfound to be of highest percentage, 26(52%) in the age group less than 1year, The least infection2 (4%) wasfound in group between7-8. There were no detectable cases of bacterial infection among the age group of9-10 years in this study.Amikacin showed efficacy in 26(96.6%) isolates of E.coli species, also showedefficacy in 16(94.1%)isolate of Klebsiella spp.Amikacin,cefepime,meropenem,gantamicin showed efficacyin 3(100%)isolate of Kluyvera ascorbate
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