<![CDATA[This study was designed to evaluate the analysis of blood samples in crime scene under different environmentalconditions. Samples are divided into four main groups depending on the source of bloodspot: Soil, Stone,Ceramic, and Water sample. Each group consisted of two sub-groups depending on weather temperature inIraq in winter and summer: 8 °C groups and 45 °C groups. Each group consisted of 32 samples. Sampleswere collected after different time (0, 2, 6, and 24) hours.The results of the current study showed variation in the ability to extract DNA depending on the surface type.In addition, DNA can be extracted from bloodstains on soil, gravel, ceramics, and water when extractedimmediately after exposure to the surface. The difference in the ability to extract DNA from bloodstainson different surfaces after two hours at 8 °C temperature, where the success rate of extraction is (25, 7.5,100, and 100%) for soil, stone, ceramics, and water samples Respectively. The ability rate to extract DNAafter two hours at 45 °C temperature, where (25, 7.5, 87.5, and 100%) for soil, gravel, ceramics, and watersamples respectively. The ability rate to extract DNA after six hours at 8 °C temperature, where (0, 12.5,37.5, and 100%) for soil, gravel, ceramics, and water samples respectively, while it was (0, 12.5, 25, and100%) for six hours samples at 8 °C temperature. After 24 hours, only water samples showed the ability toextract DNA from all samples, while no DNA was obtained from the rest of the samples.PCR amplification of the FIF2AK3 gene showed products when the DNA extracted immediately after theiradhesion to the surface of the soil while showing no products for other age groups. Also, the PCR productscan be obtained when DNA isolate immediately after exposure to the stone surface, whereas the rate PCRamplification products of DNA isolated after two hours was (37.5%) for both groups 8 °C and 45 °C., whileother age groups showing not products.The agarose gel electrophoresis of PCR products showed the possibility of FIF2AK3 gene amplificationwhen blood samples were taken immediately after exposure to the surface, whereas the ability to obtainamplification products was (100 and 87.5%) for groups 8 °C and 45 °C respectively. No products wereobtained after 6 and 24 hours. In water samples, all isolated DNA gave the product.]]>
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