<![CDATA[Pseudomonas aeruginosa is one of the most important pathogen in both hospital and community-acquiredinfections, it is a classical opportunistic pathogen, and this organism does not normally attack healthytissue, and rarely causes infection in the general population. It is possess various virulence mechanismsto adhere, colonize and biofilm formation. Samples were collected from different hospitals in Baghdad,samples were collected and categorized in to two main groups: eighty clinical samples and forty as anenvironmental samples. Forty one isolates were identified as P. aeruginosa by using conventional methodsand confirmed by VITEK-2 compact system, biofilm were detected by three methods: congo-red agar, tubemethod and microliter plate. Synergistic Effect of Amikacin and Ciprofloxacin on biofilm formation weredetected phenotypically by using microliter plate and genetically by Real-time PCR for pelA and algDGenes which are responsible for biofilm formation. The results shows an obvious differences in biofilmformation phenotypically and genotypically for the treated isolates]]>
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