<![CDATA[Klebsiella pneumoniae is an opportunistic pathogen leading to nosocomial infections with production ofbiofilm. In the present study, 73 samples were collected from patients of different ages and both genderssuffering from burn and wound injury that referred to four Hospitals in Baghdad from Aug. to Nov. 2019.All Klebsiella isolates were subjected to the primary identification; first by describing colonies grown onMacConkey agar and second microscopically after Gram staining of their cells. Then the isolates wereidentified by Vitek 2 system which insured that 60 of the isolates were found to belong to Klebsiellapneumoniae. All identified isolates were screened for ability to produce biofilm by using the Microtiterplate assay and the Congo red agar method, and the results showed that only 4 isolates (3 of wounds and1of burns) were able to form biofilms. For the importance of these four isolates as the only ones able toproduce biofilm, they were subjected to the 16S rRNA gene amplification test by using PCR technique.Results insured that they are new strains Klebsiella pneumoniae to be discovered for the first time in thisstudy. They were verified and documented by the “National Center for Biotechnology Information (NCBI)”and registered as; KPWIQ25, KPWIQ49, KPWIQ51 and KPBIQ19 strains of Klebsiella pneumoniae. Whenthe probiotic filtrates and antibiotics combination were investigated for eradication of biofilms formed bythe four pathogenic strains, results showed that such combinations had great efficiency in the eradication.]]>
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