Sensitive Simultaneous Estimation of Atorvastatin Ca in Pure and Dosage Forms Via Developed CFIA using 1,2 Naphthoquinone-4-Sulfonate as a Suitable Organic Agent

Bushra B. Qassim1 , Asmaa A. Zydan2

Indian Journal of Forensic Medicine & Toxicology
Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology

Objectives: A sensitive visible spectrophotometric method and FIA/merging zones technique was developedfor the determination of atorvastatin calcium in pure material and tablet dosage form.Methods: Atorvastatin calcium has a free carboxylic moiety in its structure, which when being deprotonatedin basic medium facilitates associated the reagent with the drug. This method was based on the formationof red colored chromogen of drug with 1,2-Naphthaquinone-4-sulfonate(NQS) in basic medium (NaOH).The absorbance of the chromogens was measured at their respective wavelengths of maximum absorbanceagainst the corresponding reagent blankResults: The red colored product is directly completed in basic medium and exhibits maximum absorptionat 525 nm. Different factors affecting the formation of the product and optimized in order to obtain the bestconditions for the experiment and its stability were studied. Method validation was done over a concentrationrange of 2-10 and 1-20 ?g/mL for batch and FIA method respectively.