<![CDATA[Background: Drug-resistant TB is the most important issue that threatens to interrupt the improvementsachieved in tuberculosis control. Multidrug-resistant (MDR) tuberculosis is defined as resistance to bothrifampicin and isoniazid.Objectives: To find molecular feature via DNA extraction for genes in charge of drug resistance in pulmonarytuberculosis patients with hyperglycemia and role of the Xpert MTB/RIF Ultra molecular test efficacy inDR-TB cases detection.Method: 106 pulmonary TB patient sputum and blood samples were collected in the Institute of Chest andRespiratory diseases in Baghdad and Medical City Hospital from December 2018 - July 2019, 52 were drugresistances TB patients. All patients were examined by conventional method such as direct examination(AFB test), Gene-Xpert test, FBS and HbA1c test were carried out for all cases.Results: GeneXpert/ultra results revealed 43 cases were Rif resistance in which it was highly significant.The GeneXpert/ultra quantity was mostly medium in the bacillary load. The Rif’s resistance cases weremostly registered in the age group (20-59) and in male population. The most frequent patient’s type was NewMDR. The GeneXpert/ultra quantity and HbA1c levels results showed that most elevated HbA1c cases hada medium to high bacillary load. The DST results were significant in which 95.3% were Rif’s resistance and62.8% were STR’s resistance. The LPA on 23 DR cases showed the following; 13 patients were MDR casesin which a deletion in the band WT8 and mutation in the MUT3 band of the gene rpoB and deletion in theinhA WT1 band of the inhA gene were the most notice.Conclusions: Molecular method had showed that mutations in the rpoB gene especially 530 and S531Lcodon are responsible for majority of RMP resistance in MTB while mutations at the -15/-16 InhA codonand 315 of KatG codon responsible for most INH-resistance cases.]]>
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