The chemical composition, antioxidant, antifungal, and physiological effects of commercial Melaleuca leucadendron LINN. (Kayu Putih) oil from Sukun, East Java, Indonesia was investigated in this study. GC-MS analysis showed the majority compounds of this oil are mixtures of monoterpenes and sesquiterpenes, which 1,8-cineole (53.90%) was the most abundant compound in this oil. In vitro antioxidant assay showed this essential oil possessed antioxidant activity (IC50: 4.24mg/ml). In vitro antifungal assay showed relatively strong antifungal activity of this oil against plant pathogenic fungi of F. oxysporum (IC50: 0.44mg/ml), T. cucumeris (IC50: 0.97mg/ml) and R. oryzae (IC50: 7.71mg/ml). The investigation on the effect of the odor of M. leucadendron oil in this study also showed functional fragrance of this oil for human physiological behavior controlling.
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