Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science)
Vol 26, No 2 (2016)

Sintesis protein mikroba dan aktivitas selulolitik akibat penambahan level zeolit sumber nitrogen slow release pada glukosa murni secara in vitro

Al Qori'ah (Fakultas Peternakan dan Pertanian Universitas Diponegoro)
Surono Surono (Fakultas Peternakan dan Pertanian Universitas Diponegoro)
Sutrisno Sutrisno (Fakultas Peternakan dan Pertanian Universitas Diponegoro)



Article Info

Publish Date
29 Sep 2016

Abstract

This research aimed to determine the effect of the level of zeolites as slow release nitrogen in pure glucose on microbial protein synthesis and cellulolytic activity. The material used were zeolite of clinoptilolite type with size 40-60 mesh, urea, pure glucose, cow's rumen fluid from RPH Ungaran. The research used completely randomized design (CRD) with 5 treatments and 4 replications. The treatments were T1, T2, T3, T4 and T5 = pure glucose + additional level 2%, 4%, 6%, 8% and 10% of zeolite slow release respectively. Variables observed were microbial protein synthesis and cellulolytic activity. The results showed that the treatment significantly (P<0.05) increased microbial protein synthesis and cellulolytic activity. Results of protein synthesis from higher to lower number were T2 (59.50 mg/ml), T3 (55.58 mg/ml), T4 (47.55 mg/ml), T5 (42.82 mg/ml) and T1 (31.68 mg/ml) respectively. Results of cellulolytic activity from higher to lower number were T5 (3.55 mol ml-1 min-1), T4 (2.36 mol ml-1 min-1), T2 (2.23 mol ml-1 min-1), T3 (2.16 mol ml-1 min-1) and T1 (2.09 mol ml-1 min-1) respectively. The results of microbial protein synthesis and cellulolytic activity at each treatment were in normal level. The addition of the zeolite level as slow release nitrogen source up to 4% increase microbial protein synthesis. Keywords : Zeolites, microbial protein synthesis and activity of cellulolytic

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Journal Info

Abbrev

jiip

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Other

Description

Jurnal Ilmu-Ilmu Peternakan (JIIP) is a journal published and managed by the Faculty of Animal Husbandry Universitas Brawijaya. JIIP is a peer-reviewed journal published three times a year. JIIP now actively using Open Journal System (OJS). JIIP mediates the dissemination of researchers various ...