METAMORFOSA Journal of Biological Sciences
Vol 3 No 2 (2016)

PENYELAMATAN EMBRIO Dendrobium anosmum Lindl. MELALUI KULTUR IN VITRO

Putu Yuni Astriani Dewi (Unknown)
Ida Ayu Astarini (Unknown)
Eniek Kriswiyanti (Unknown)



Article Info

Publish Date
23 Sep 2016

Abstract

The aim of the research is to analyse germination percentage, effect of coconut water addition in the media to number of last stage embryos and development of final stage D. anosmum Lindl. embryo. Research was conducted at Plant Structure and Development of Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences University of Udayana and UPT. Balai Benih Induk Tanaman Pangan dan Hortikultura Provinsi Bali from February 2015 to July 2015. Three type of media were employed, i.e. PDA, Organic, MS. Three different concentration of coconut was added to media, i.e. 0 ml/L 50 ml/L, 100 ml/L. Results showed that seed D. anosmum Lindl. on PDA, Organic and MS has not germinated yet on all media, only swollen cells observed. Seed dormancy and maturity of seed were the main factors affecting seed germination.  Addition of 100 ml / L coconut water in MS showed that media is capable to increase the formation of final stages embryos (> 32 cells). Embryo development on PDA and Organic largely showed cell stage embryos 1 to 8 cells, while MS media already showed embryo development stages of > 32 cells.

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Journal Info

Abbrev

metamorfosa

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Description

METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of ...