Simultaneous Determinations of Aflatoxins B , B , G , And G Using Hplc with Photodiode-Array (PDA) Detector in Some Foods Obtained from Yogyakarta, Indonesia

Abdul Rohman (Analytical Chemistry Laboratory, Department of Pharmacological Chemistry, Faculty of Pharmacy, Gadjah Mada University, Sekip, Yogyakarta 55281)
Triwahyudi Triwahyudi (Integrated Research and Testing Laboratories, Gadjah Mada University, Sekip, Yogyakarta 55281)

agriTECH
Vol 28, No 3 (2008)

A method has been validated for simultaneous determinations of aflatoxins B , B , G and G  using HPLC with photodiode­array (PDA) detector in some foods obtained from Yogyakarta. An optimum condition is reached using column ResolveTM C   (150 mm x 3.9 mm i.d; 5 μm). The mobile phase used is a mixture of methanol­acetonitrile­water (17: 1819: 63 v/v) delivered isocratically at 1.2 ml/min. All aflatoxins were detected at 365 nm. The samples of food (corn, rice,and pea nuts) were obtained from local markets and super markets in Yogyakarta. The sample was prepared by solid phase extraction using a Florisil cartridge.The method revealed a good linearity for all aflatoxins evaluated by determination coefficients (r2) > 0.99. The recoveries of aflatoxins B , B , G and G  are 84.78; 82.14; 85.29; and 89.06 %, respectively. The detections limits are 13.20;9.36; 7.54; and 6.75 μg/L. The RSD values of intra­day precisions are 0.38; 1.27; 3.09; and 1.76 %, respectively. Thevalidated method has been used to quantify the level of flatoxins in foods.