Possible Involvement of A Monophenol Monooxygenase in A-Factor-Dependent Yellow Pigment Production in Streptomyces griseus

Jumeri Jumeri (Jurusan Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Universtas Gadjah Mada, Yogyakarta)
Yasuo Ohnishi (Department of Biotechnology, Graduate School of Agriculture and Life Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan)
Sueharu Horinouchi (Department of Biotechnology, Graduate School of Agriculture and Life Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan)

agriTECH
Vol 21, No 1 (2001)

A-factor (2-isocapryloyl-3R-hydroxymethil-γ-butyro-lactone) is an essential hormonal regulator for morphological development, as well as streptomycin and yellow pigment productions in Streptomyces griseus. By UV-treatment, three mutants that were deficient in yellow pigment production but showed normal morphologiical development and strptomycin production were obtained. A 7.5 kb-DNA fragment, which restored the yellow pigment production in one of the mutants, was shotguncloned from a chromosomal DNA of wild-type SS. griseus. Sequence analysis of this fragment showed that it contained 6 complete open reading frmaes including melC1 and melC2. MelC2 and MelC1 have high sequence similarity to monophenol monooxygenases and co-factors of the enzyme, respectively. Because a melCC2-disrupted strain showed yellow pigment deficient phenotype, it was probable that the monophenol monooxygenase homologue is involved in the biosynthesis of the yellow pigment.