Bumi Lestari
Vol 13 No 2 (2013)

PENGARUH TOTAL FENOLTERIPANG PASIR (Holothuria scabra) TERHADAP RESPON IMUN NON SPESIFIK IKAN MAS (Cyprinus carpio)

Achmad Suhermanto (Unknown)



Article Info

Publish Date
01 Aug 2013

Abstract

AbstractThe aim of the study was to know administration and applications of optimal doses of seacucumber total phenol to increase nonspecific immune response of Carp. Bioactive componentextraction was done by methanol and for further fractionation was dissolved with ethylacetate (v/v). Total phenol identification on ethyl acetate fraction was done to utilize UVVisspectrophotometer and infrared. Total phenol produced was tested on carp withintraperitonial injection, the concentration of 0; 0,09; 0,18; and 0,27 mg phenols/kg fish..Challenge be done utilizes A. hydrophila (107cell/ml) with immertion method. Statisticalanalysis involved one-way analysis of varians (ANOVA) by minitab 14. The level ofsignificance were expressed as P-value less or greater than 0.05.The result of hematological parameters showed that hematocrit (PVC), hemoglobin,erythrocytes, lymphocyte pre infection was increased significantly and post-bacterial wasdecreased significantly (p<0.05). Leukocytes, Neutrophils pre-and post-infection weresignificantly increased (p<0.05). Eosinophils, monocytes pre-and post bacterial infectionwere not differ significantly between treatment (p<0.05).

Copyrights © 2013






Journal Info

Abbrev

blje

Publisher

Subject

Agriculture, Biological Sciences & Forestry Humanities Earth & Planetary Sciences Energy Environmental Science

Description

Bumi Lestari Journal of Environment (p-ISSN: 1411-9668; e-ISSN: 2527-6158) is a peer-reviewed journal, presents original research articles and letters in all areas of environmental studies and environmental sciences (biotic, abiotic and social/cultural). This journal has been indexed by cabdirect ...