Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

2.024

P-Index

This Author published in this journals

All Journal Jurnal Akuakultur Indonesia Bumi Lestari JURNAL ILMIAH PLATAX Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Acta Aquatica: Aquatic Sciences Journal Jurnal Biologi Tropis Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Pari Jurnal Airaha Ranah Research : Journal of Multidisciplinary Research and Development

Suhermanto, Achmad

Politeknik Kelautan Dan Perikanan Karawang

Author-ID : 682134

Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Public Health Social Sciences Transportation Other

Published : 14 Documents Claim Missing Document

Claim Missing Document

Articles

1 2

PEMBERIAN TOTAL FENOL TERIPANG PASIR (Holothuria scabra) UNTUK MENINGKATKAN LEUKOSIT DAN DIFERENSIAL LEUKOSIT IKAN MAS (Cyprinus carpio) YANG DIINFEKSI BAKTERI Aeromonas Hydrophila Achmad Suhermanto; Sri Andayani; M Maftuch
Jurnal Kelautan Vol 4, No 2: Oktober (2011)
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jk.v4i2.879

Freshwater fishery commodities are most likely to be increased production of carp (Cyprinus carpio). Intensification of cultivation which raises new problems with fish disease outbreaks caused by Aeromonas hydrophila. Providing of immunostimulatory bioactive compounds of sea cucumbers (Holothuria scabra) can enhance non-specific immune responses in the goldfish as a mechanism of defense against disease. The purpose of this study is to determine the role and optimal dose of total phenols sea cucumber in enhancing non-specific immune response seen from the hematology parameters Extraction of bioactive components from sea cucumber prepared by using methanol and fractionated with ethyl acetate solvent (v/v). Identification of total phenols in ethyl acetate fraction performed using UV-Vis spectrophotometer and infrared. Total phenol produced was tested on carp with intraperitonial injection, the concentration of 0.09, 0.18 and 0.27 mg phenol/kg fish. Challenge test was done by using the Aeromonas hydrophila (107 cells/ml) through the immersion method. The observations included hematological parameters and total plasma proteins.The observations of hematological parameters that Leukocytes, Neutrophils pre-and post-infection were significantly increased. Lymphocyte pre infection was increased significantly and post-bacterial infection was decreased significantly. Eosinophils, Monocytes pre-and post-bacterial infection were not differ significantly between treatment. The use of phenolic compounds at 0.09 mg / kg can be increase of non-specific immune response in carp (Cyprinus carpio). Key Words : Total phenol, Aeromonas hydrophila, Cyprinus carpio, nonspecific immune response

PENGARUH TOTAL FENOLTERIPANG PASIR (Holothuria scabra) TERHADAP RESPON IMUN NON SPESIFIK IKAN MAS (Cyprinus carpio) Achmad Suhermanto
Bumi Lestari Journal of Environment Vol 13 No 2 (2013)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

AbstractThe aim of the study was to know administration and applications of optimal doses of seacucumber total phenol to increase nonspecific immune response of Carp. Bioactive componentextraction was done by methanol and for further fractionation was dissolved with ethylacetate (v/v). Total phenol identification on ethyl acetate fraction was done to utilize UVVisspectrophotometer and infrared. Total phenol produced was tested on carp withintraperitonial injection, the concentration of 0; 0,09; 0,18; and 0,27 mg phenols/kg fish..Challenge be done utilizes A. hydrophila (107cell/ml) with immertion method. Statisticalanalysis involved one-way analysis of varians (ANOVA) by minitab 14. The level ofsignificance were expressed as P-value less or greater than 0.05.The result of hematological parameters showed that hematocrit (PVC), hemoglobin,erythrocytes, lymphocyte pre infection was increased significantly and post-bacterial wasdecreased significantly (p<0.05). Leukocytes, Neutrophils pre-and post-infection weresignificantly increased (p<0.05). Eosinophils, monocytes pre-and post bacterial infectionwere not differ significantly between treatment (p<0.05).

VIRULENCE GENE PROFILING AND PATHOGENICITY OF Streptococcus agalactiae ISOLATED FROM TILAPIA, Oreochromis niloticus FARMS IN INDONESIA Sukenda Sukenda; Achmad Suhermanto; Muhammad Zairin Jr.; Angela Mariana Lusiastuti; Sri Nuryati; Dendi Hidayatullah
Indonesian Aquaculture Journal Vol 16, No 2 (2021): (December, 2021)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.16.2.2021.119-125

Streptococcosis caused by Streptococcus agalactiae has become a major disease problem in tilapia culture in Indonesia. This study aimed to detect virulence genes of S. agalactiae during streptococcosis disease outbreaks in several tilapia farms in Indonesia and evaluate the correlation between biotype and virulence genes to bacterial virulence. The presence of virulence genes was determined in 10 strains of S. agalactiae isolated from farm-raised tilapia. Polymerase chain reaction (PCR) protocol was used to determine genes for cylE, hylB, lmb, bib A, PI-2b, fbs A, fbs B, gap, PI-1, and cfb in the template DNA. Pathogenicity test was carried out by intraperitoneal injection of 24 hour-cultured S. agalactiae to tilapia with 108 CFU/fish. Four isolates have seven of virulence genes (cylE, hylB, bibA, PI-2b, fbs A, fbs B, and gap genes), three isolates have six virulence genes (hylB, bib A, fbs A, fbs B, gap, cfb genes), one isolate has four virulence gene (hyl B, bib A, fbs, and cfb genes), and one isolate has one virulence gene (PI-2b gene). None of the isolates has lmb or PI-1 genes. Bacteria with more virulence genes showed higher pathogenicity post injection. Mortality of tilapia injected with b-hemolytic bacteria was 100% within the period of 14-19 hours, while non-hemolytic bacteria was 53.3%-86.6% on 14 days post-injection. Pathological changes associated with the infection by either isolate included melanosis, slow response, anorexia, ocular opacity, gasping, erratic, C-shape, and whirling. It can be concluded that S. agalactiae with more virulence genes show a higher level of pathogenicity. The presence of a virulent gene has the potential to be used as a basis for selecting candidate isolates and designing vaccine compositions as an effort to prevent streptococcosis infection in tilapia in Indonesia.

POLA INFEKSI Streptococcus agalactiae STRAIN NP105O DAN N14G PADA IKAN NILA (Oreochromis niloticus) Achmad Suhermanto; Suhermin Suhermin; Ridwan Ridwan; Indri Astuti; Iis Nurmawanti
Jurnal Riset Akuakultur Vol 15, No 1 (2020): (Maret, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Infeksi Streptococcosis yang disebabkan oleh bakteri patogen Streptococcus agalactiae dengan karakteristik strain berbeda menjadi permasalahan utama pada budidaya ikan nila. Penelitian ini bertujuan untuk menelaah pola infeksi bakteri S. agalactiae strain NP105O dan N14G, melalui performa organ target, gejala klinis, serta hematologi ikan nila Oreochromis niloticus. Karakterisasi S. agalactiae berdasarkan pada SNI dan API 20 STREP, uji pertumbuhan bakteri dilakukan dengan metode total plate count (TPC). Pengujian eksistensi bakteri dengan cara menginjeksikan S. agalactiae secara intraperitoneal (IP) dengan konsentrasi 107 CFU.mL-1, dan pengamatan dilakukan dengan pengambilan darah dan dikultur di media BHIA. Hasil uji biokimia dan konfirmasi menggunakan API 20 STREP menunjukkan bahwa isolat terkonfirmasi positif sebagai S. agalactiae, dan NP105O dideteksi sebagai bakteri â-hemolitik. Pertumbuhan bakteri NP105O lebih cepat daripada N14G, namun eksistensi di darah masing-masing selama 72 jam dan 24 jam. Hasil pengamatan performa darah menunjukkan bahwa glukosa dan leukosit mengalami peningkatan signifikan masing-masing 53,5 ± 2,12 mg.dL-1 dan 6,51 ± 0,89 (105 sel.mm-3), sedangkan hematokrit dan eritrosit mengalami penurunan signifikan (P<0.05) masing-masing 21,10 ± 0,07% dan 14 ± 4,5 (105 sel.mm-3) pascainjeksi S. agalactiae. Gejala klinis pascainfeksi berupa melanosis, respons lambat, anorexia, ocular opacity, purulens, unilateral atau bilateral eksoptalmia, gasping, erratic, C-shape, dan whirling. Pola infeksi S. Agalactiae strain NP105O dan N14G berbeda pada ikan nila, dan sangat dipengaruhi oleh keberadaan bakteri pada organ ginjal, otak, dan mata.Streptococcosis infection caused by different strains of pathogenic bacteria Streptococcus agalactiae has been a major problem in tilapia culture. This research aimed to examine the patterns of S. agalactiae infection of NP105O and N14G strains, through targeted organ performance, clinical symptoms and hematological signs of tilapia, Oreochromis niloticus. The characterization of S. agalactiae was based on SNI and API 20 STREP. Bacterial growth test was carried out using the total plate count (TPC) method. Bacterial infection was performed by injecting S. agalactiae intraperitoneally (IP) with a concentration of 107 CFU.mL-1, and the observations were carried out by extracting and culturing the fish blood in BHIA media. The result of the biochemical test and API 20 STREP confirmed that the isolates was identified as S. agalactiae and NP105O strain was detected as â-hemolytic bacteria. The growth of NP105O strain was faster than N14G strain, where their clear presence in blood was observed at 72 and 24 hours, respectively. The result of hematological parameters showed that glucose and leukocytes increased significantly with values of 53.5 ± 2.12 mg.dL-1 and 6.51 ± 0.89 (105 cell.mm-3), respectively. On the other hand, hematocrit and erythrocytes decreased significantly (P<0.05) 21.10 ± 0.07% and 14 ± 4.5 (105 cell.mm-3) post-S. agalactiae injection. Clinical signs post-infection consisted of melanosis, slow response, anorexia, ocular opacity, purulence, unilateral or bilateral exophthalmos, gasping, erratic movement, C-shape and whirling. NP105O, and N14G strains show different patterns of infections on tilapia and strongly influenced by the presence of bacteria in the kidneys, brain, and eyes.

TOKSISITAS SEL UTUH DAN EXTRACELLULAR PRODUCT (ECP) Streptococcus agalactiae β-HEMOLITIK DAN NON-HEMOLITIK PADA IKAN NILA (Oreochromis niloticus) Achmad Suhermanto; Sukenda Sukenda; Muhammad Zairin Jr.; Angela Mariana Lusiastuti; Sri Nuryati
Jurnal Riset Akuakultur Vol 13, No 4 (2018): (Desember 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Bakteri Streptococcus agalactiae tipe β-hemolitik dan non-hemolitik menjadi agen penyebab infeksi streptococcosis yang mengakibatkan kematian dan kerugian besar pada budidaya ikan nila. Penelitian ini bertujuan untuk membandingkan toksisitas sel utuh dan extracellular product (ECP) bakteri b-hemolitik dan non-hemolitik yang diinjeksikan pada ikan nila. Karakterisasi S. agalactiae berdasarkan SNI dan API 20 STREP, serta pemisahan protein dengan metode SDS-PAGE. Pengujian toksisitas dilakukan dengan cara menginjeksikan sel utuh dan ECP S. agalactiae secara intraperitoneal (IP) dengan dosis 0,1 mL ekor-1. Hasil uji biokimia, dan konfirmasi dengan API 20 STREP menunjukkan bahwa semua isolat positif S. agalactiae. Fraksinasi protein pada sel utuh bakteri diperoleh pita protein masing-masing sebanyak sembilan dan tujuh pita pada tipe β-hemolitik dan non-hemolitik. Fraksinasi ECP teridentifikasi pada β-hemolitik sebanyak tujuh pita dan non-hemolitik empat pita protein. Konsentrasi protein sel utuh dan ECP b-hemolitik lebih besar dibandingkan bakteri non-hemolitik. Gejala abnormalitas lebih cepat terjadi pada ikan nila yang diinjeksi ECP bakteri b-hemolitik dan berbanding lurus dengan kematian sebanyak 91%-100% pada jam ke-13 pascainjeksi. Hasil ini menunjukkan bahwa ECP bakteri S. agalactiae β-hemolitik lebih virulen dibandingkan tipe non-hemolitik. Hingga akhir pemeliharaan tidak ada kematian pada ikan yang diinjeksi sel utuh bakteri S. agalactiae b-hemolitik dan non-hemolitik. Studi histopatologi ikan yang diinjeksi ECP S. agalactiae pada organ hati, limpa, otak, dan ginjal menunjukkan adanya kongesti, hemoragi, dan nekrosis.The β-hemolytic and non-hemolytic biotype of Streptococcus agalactiae are the agents that cause streptococcosis infection which resulted in high mortality and major losses in tilapia culture. This study aimed to compare the toxicity of whole cell and extracellular product (ECP) b-hemolytic and non-hemolytic bacteria from injected tilapia. Characterization of S. agalactiae was based on SNI and API 20 STREP and protein separation by SDS-PAGE method. Toxicity test was carried out by injecting whole cells and ECP S. agalactiae intraperitoneally with a dose of 0.1 mL fish-1. The results of biochemical tests, with confirmation by API 20 STREP showed that all isolates were positive for S. agalactiae. Protein fractionation of whole bacterial cells obtained as many as nine and seven bands of protein in b-hemolytic and non hemolytic biotype, respectively. ECP fractionation was identified in β-hemolytic biotype as many as seven bands and four protein bands in non-hemolytic. The whole cell protein concentration and ECP β-hemolytic were higher than non-hemolytic bacteria. Symptoms of abnormalities occurred faster in tilapia which was injected with ECP b-hemolytic bacteria and had positive correlation with 91%-100% mortalities at the 13th hours post-injection. This results indicated that ECP of S. agalactiae β-hemolytic are more virulent than non-hemolytic. Until the end of the trial, there were no deaths in fish injected with whole cells of b-hemolytic and non-hemolytic S. agalactiae. Histopathological studies of ECP-injected fish S. agalactiae in the liver, spleen, brain, and kidneys showed congestion, hemorrhage, and necrosis.

The Pemberian Keterampilan Budi Daya Ikan dalam Ember (Budikdamber) di Kelurahan Saoka, Tanjung Kasuari, Kota Sorong Agung Setia Abadi; Ernawati Ernawati; Intanurfemi Bacandra; Hismayasari Hismayasari; Mohammad Sayuti; Achmad Sofian; Kadarusman Kadarusman; Achmad Suhermanto; Saidin Saidin
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 8 No. 2 (2022): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.8.2.175-181

The Covid-19 pandemic that occurred in Indonesia and even the world caused a decline in people’s income because several work sectors experienced disruption. The aim of providing fish farming skills in buckets (Budikdamber) will be an alternative source of income for the people of Saoka Village, Tanjung Kasuari, Sorong City in the midst of pandemic. A site survey was conducted to determine the place and to find participants in the activity. The service process is carried out in two stages, namely The provision of Budikdamber material is carried out online, while the practice of making Budikdamber is carried out offline. A total of 1200 catfish were stocked in 6 buckets which reselted in a total fish survival rate of 60%. The results obtained by catfish that are kept are able to live and grow even though there is death in the fish. After four months of rearing. The fish can be sold whit a yield of 112 kg whit a selling price of IDR 35,000/kg, whit a total income of IDR 3,920,000. This shows that the tarining is able to provide an alternative source of income for the people of Saoka Village.

Karakteristik Bakteri Streptococcus agalactiae NP104O, S01-196-16 dan NMbO Penyebab Streptococcosis pada Ikan Nila (Oreochromis niloticus) Achmad Suhermanto; Herdianto T; Suhermin; Ridwan; Nurmawanti I
Jurnal Airaha Vol 8 No 02: DEC 2019
Publisher : Politeknik Kelautan dan Perikanan Sorong, Indonesia

Failure of tilapia culture in Indonesia in the hatchery, nursery and rearing segments mostly is caused by Streptococcus agalactiae infection. This study aimed to detect the characteristics of bacteria S. agalactiae NP104O, S01-196-16 and NMbO which infect tilapia in aquaculture. Bacterial isolation was carried out during an outbreak of streptococcosis, further analysis based on morphological and biochemical characters. The pathogenicity test was done by injecting bacteria intraperitoneally at a dose of 107 CFU fish-1. The results showed that all isolates was cocci, gram positive, negative catalase and negative oxidase. The three isolates had different concentrations and growth patterns until end of the observation. Pathogenicity test results obtained all fish injected with NP104O, S01-196-16 and NMbO bacteria appeared as clinical symptoms of melanosis, unilateral or bilateral exophthalmos, turbidity in the eye, curved vertebrae forming the letter C, erratic, and whirling. Hopefully, the results of this study can be used as a reference for prevention of streptococcosis infections in tilapia.

The potential of Barringtonia asiatica Biopesticide from Papua to Eradicate Pests in Aquaculture Achmad Suhermanto; Fabian Ardianta; Murtihapsari Murtihapsari; Achmad Sofian
Jurnal Airaha Vol 11 No 01: June 2022
Publisher : Politeknik Kelautan dan Perikanan Sorong, Indonesia

Saponins are compounds derived from plants that are currently widely used in aquaculture for aquatic pest control. In this study, potential saponin components extracted from fish poison tree (Barringtonia asiatica), which is widely dispersed in tropical areas worldwide, were characterized. Saponin properties were obtained from seeds and leaves by extraction and spectrophotometric methods. To test its properties, four different concentrations of saponin extracts, namely 10 ppm, 20 ppm, 30 ppm, and 40 ppm, were applied in four trials containing 20 tilapias each. An unexpected result was obtained and proved that the saponins extracted from the seeds of the fish poison tree proved to be significantly more effective than the saponins extracted from the peel of its fruit to eradicate pest in pond. The results also revealed that the lethal dose of saponins reached higher level at a concentration of 40 ppm. It can be concluded that fish poison tree as a poisonous tree plays an important role in ensuring the sustainability of saponin stocks. The use of natural materials such as fish poison tree as a biopesticide has the potential to minimize environmental damage and reduce costs for aquaculture.

A Study of The Treatment of Larvae Vannamei Shrimp (Litopenaeus vannamei) In Banyuwangi Ramli, Taufik Hadi; Suhermanto, Achmad; Andi, Rudi; Sangkia, Frederik Dony
Jurnal Ilmiah Platax Vol. 11 No. 1 (2023): ISSUE JANUARY-JUNE 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i1.47714

Vannamei shrimp (Litopenaeus vannamei) is a brackish water commodity that is currently in great demand and is a leading producer of the aquaculture sector in Indonesia. This is because some of the advantages possessed by the vannamei shrimp, among others, can be maintained with a high stocking density, rapid growth, has a high resistance to disease and environmental changes. The mating process occurs through four stages, namely approach, pursuit, pride, and mating. This process can be known by looking at the behavior of the male parent who swims to follow the female parent. They both looked like a chase. Then the male parent swims parallel to the female and turns her body towards the female shrimp's belly. After that, the male grabs the female and releases sperm that attach to the thellycum. In the maintenance of larvae that must be considered is the management of water quality and feed management. This is because water is a living medium for aquatic organisms, so it determines the survival of larvae. During the period of high maintenance and market, demand continues to increase where the shrimp farming process includes the hatchery stage to enlargement. Vannamei shrimp hatchery activities are inseparable from the availability of quality fry

Initial study of population and microhabitat of the Banggai cardinalfish (Pterapogon kauderni) in Bilalang Bay, Banggai Regency Samsu Adi Rahman; Herdiyanto Djiada; Achmad Suhermanto; Muhammad Safir
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica, Vol. 11: No. 3 (December, 2024)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v11i3.16488

The Banggai cardinalfish is an endemic fish in Banggai waters which is experiencing a population decline caused by excessive exploitation and degradation of its microhabitat. This research aims to determine the population of the Banggai cardinalfish and its microhabitat in Bilalang Bay. The research was conducted from January to February 2023 in Bilalang Bay, Banggai Regency, Central Sulawesi. Research data collection used the Underwater Visual Survey (UVS) method using a 20mx5m belt transect (2.5m left and right of the transect rope) at five observation stations. Observations made included recruits (25 mm) Total Length, juveniles (25-60 mm), and adults (60 mm). The results of observations of the population and density of the Banggai cardinalfish differ based on five observation stations. The highest population of the Banggai cardinalfish is found at location II with a total population of 4,412 fish, consisting of 2,510 adult size fish, 1,800 juvenile size fish, and 102 recruit size fish, with a density of adults 21.1 ind/m2, juvenile density 8 ind/m2, and recruit density 0.5 ind/m2. Meanwhile, the highest population based on size is adult size. The recruitment stage of the Banggai cardinalfish is only found in stations 1, 2, and 3, with microhabitats of sea urchins and anemones. The Banggai cardinal fish has the highest population at station 2.Keywords: Endemic; Banggai cardinalfish; Microhabitat; Population; Bilalang Bay

Co-Authors Achmad Sofian Agung Setia Abadi Andi, Rudi Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anton Anton Ardana Kurniaji Atiqoh Zummah Budiyati Budiyati, Budiyati Dendi Hidayatullah Ernawati Ernawati Ernawati Ernawati Fabian Ardianta Herdianto T Herdiyanto Djiada Hismayasari Hismayasari IIN SITI DJUNAIDAH Iis Nurmawanti Indri Astuti Intanurfemi Bacandra Kadarusman Kasful Anwar Maftuch Maftuch Muhammad Safir Muhammad Zairin Jr. Muhammad Zairin Jr. Mukhaimin, Iman Mulyono, Mugi Murtihapsari Murtihapsari Nur Maulida Safitri Nurmawanti I Poltak, Hendra Puspitasari, Asthervina Widyastami Putri Renitasari, Diana Rahardjo, Sinung Ramli, Taufik Hadi Rasnijal, Muhammad Ridwan Ridwan Ridwan Ridwan, Ridwan Rizkiah, Riza Saidin Saidin Samsu Adi Rahman Sangkia, Frederik Dony Sayuti, Mohammad Sektiana, Sinar Pagi Sri Andayani Sri Nuryati Suhermin Suhermin Suhermin Sukenda Sukenda Sukenda Sukenda Valentine, Riris Yuli Violando, Wiga Alif Wesy Jufan Galvanis

Title

Found 14 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 14 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 14 Documents
Search