Samakia: Jurnal Ilmu Perikanan
Vol 10 No 2 (2019): Samakia: Jurnal Ilmu Perikanan

Identification of Vibrio isolated from Mangrove Sediment Near to Vannamei Ponds

Ery Gusman (Universitas Borneo Tarakan)



Article Info

Publish Date
27 Oct 2019

Abstract

This study aims to identify the vibrio in mangrove forest areas near to the location of vannamei ponds, especially in sediment substrates. The research is at the Mangunharjo sub-district in the Mayangan District, Probolinggo City, East Java Province. The time of study is august and September 2019. Isolation of bacteria being done with total plate count procedure on Thio Sulfate Citrate Bile Sucrose (TCBS) Agar, with pour plate method. Morphology characterization and the biochemical test was done to identify the bacteria. There are three points of the sampling area, with each area distance from 100 to 500 meters. This location choosing based on purposive sampling method, which main reason is the site must have mangrove with good vegetation and variation. It is transected by 5m x 5m, 100-gram sediment at 30-40 cm depth taken randomly using sediment scoop. Three samples of each sampling area chosen randomly at three different locations, then mixed. The result showed each deposit from different sampling locations having bacteria colony with color are yellow, green, and green blueish. These colony of bacteria in this sediment suspected as v. harveyii, v. alginolyticus, v. fischery dan v. mimicus.

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Journal Info

Abbrev

JSAPI

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Other

Description

Samakia Journal is one means of disseminating information on research results and science and technology advancement in fishery field managed by Faculty of Science and Technology University Ibrahimy. Since 2010, journal issuance is done 2 times a year, ie in February and August. Since 2016 the ...