Analisis Filogeni Virus Newcastle Disease Isolat Bali Tahun 2013 Sampai 2014 Berdasarkan Sekuen Daerah Pemotongan Protein Fusion

Fedri Rell (Laboratorium Mikrobiologi, Program Studi Kedokteran Hewan Fakultas Kedokteran Universitas Hasanddin, Jl. Perintis Kemerdekaan kampus Tamalanrea Km. 10 Makassar, Indonesia 90245)
Anak Agung Ayu Mirah Adi (Laboratorium Patologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar Bali, Indonesia 80225.)
I Gusti Ngurah Kade Mahardika (Laboratorium Virologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. Raya Sesetan, Gg. Markisa No. 6, Denpasar, Bali)

Buletin Veteriner Udayana
Vol. 13 No. 1 Pebruari 2021

Newcastle disease is a contagious disease caused by the Avian paramyxovirus serotype 1 virus, which infected the poultry. The aims of this study were conducted to phylogenetic analysis of the fusion protein cleavage site of Newcastle disease virus found in a chicken farm in the province of Bali throughout the year 2013 to 2014. There are four isolates from sick chicken’s cases/death suspected of being infected by the Newcastle disease virus. The sequence of the protein fusion was aligned and edited using MEGA5. The amino acid sequence of the F cleavage site of all isolates was 112R-R-Q-K-R-F117 and analysis of phylogeny tree, which justified that all four are virulent of Newcastle disease virus. It is concluded that four Bali isolates in 2013 to 2014 under this study are Newcastle disease virus of genotype VII.