Uji Aktivitas Antioksidan Daging Buah Bisbul dan Batang Buah Naga Merah Menggunakan Metode 1,1-Diphenyl-2-Picrylhydrazin

Ietje Wientarsih (Laboratorium Farmasi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor Jln. Agathis Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia 16680)
Bayu Febram Prasetyo (Laboratorium Farmasi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor Jln. Agathis Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia 16680)
Ahmad Kurniawan (Sarjana Kedokteran Hewan, Fakultas Kedokteran Hewan, Institut Pertanian Bogor Jln. Agathis Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia 16680)
Fikri Hanifah (Departemen Klinik, Reproduksi, dan Patologi Fakultas Kedokteran Hewan, Institut Pertanian Bogor Jln. Agathis Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia 16680)

Jurnal Veteriner
Vol 21 No 4 (2020)

Bisbul (Diospyros discolor Willd.) is a plant of the Ebenaceae family and red dragon (Hylocereus polyrhizus) is a plant of the Cactaceae family. Bisbul and red dragon have bioactive compounds are flavonoids that act as antioxidants. The purpose of this study was measure antioxidant activity from ethanol extract of bisbul pulp and ethanol extract of red dragon fruit stem by DPPH method (1,1-diphenyl-2-picrylhydrazin). Extraction by maseration method and using 96% ethanol as solution. Antioxidant activity assay using the immersion method of DPPH measured absorption at a wavelenght 517 nm and compared with vitamin C. The result showed that the ethanol extract of bisbul pulp (IC50 46.00 ìg/mL) more strong antioxidant activity compared the ethanol extract of red dragon fruit stem (IC50 1020.96 ìg/mL).