Jurnal Biologi Tropis
Vol. 20 No. 3 (2020): September - Desember

Antimicrobial Activity of Pome granataum’s Endophytic Bacteria Against Pathogenic Microbe

Putri Alfianti Sriwinahyu (Universitas Mataram)
Prapti Sedijani (Universitas Mataram)
Lalu Zulkifli (Universitas Mataram)



Article Info

Publish Date
26 Nov 2020

Abstract

White pomegranate (Punica granatum L.) is one of potential plant that can be use as an antimicrobial. The objective of this study was to determine antimicrobial activity of endophytic bacteria from white pomegranate (Punica granatum L.) in inhibiting the growth of pathogenic microbia (Staphylococcus aureus, Streptococcus viridans, Shigella dysentriae, Klebsiella pneumoniae and Fusarium sp. fungi). The stages of the research were isolation endophytic bacteria, antimicrobial assay, and characterization of potential endophytic bacterial. Endophytic bacterial were isolated from bark, leaves, flower, and fruit peel of white pomegranate taken from Gebang Baru, Mataram District. Antimicrobial assay was then performed using agar diffusion method, using supernatant of endophytic bacteria, water as negative control or ciprofloxacin as positive control. The potential isolates were characterized using Gram staining and biochemical test. The results showed that 2 endophytic bacterial isolates (DNX2 and BNG1) show strong activity against S. aureus, 3 endophytic bacterial isolates (BTG1, DNX11, and BNG1) show strong activity against S dysentriae, and 1 isolate KLBX11 shows medium activity against Fusarium sp. fungi. Those isolates are gram-possitive with bacill-shaped and produce spores. It can be concluded that it is possible to obtain potential endophytic bacterial from white pomegranate are potential as an alternative source for antimicrobial compounds.

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Journal Info

Abbrev

JBT

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Jurnal Biologi Tropis (ISSN Cetak 1411-9587 dan ISSN Online 2549-7863) diterbitkan mulai tahun 2000 dengan frekuensi 2 kali setahun oleh Program Studi Pendidikan Biologi PMIPA FKIP Universitas Mataram, berisi hasil penelitian dan ulasan Ilmiah dalam bidang Biologi ...