ANNALES BOGORIENSES
Vol 24, No 2 (2020): Annales Bogorienses

Catechin Contents, Antioxidant and Antibacterial Activities of Different Types of Indonesian Tea (Camellia sinensis)

Isa Nuryana (Indonesian Institute of Sciences)
Shanti Ratnakomala (Research Center for Biotechnology, Indonesian Institute of Sciences)
Fahrurrozi Fahrurrozi (Research Center for Biotechnology, Indonesian Institute of Sciences)
Ario Betha Juanssilfero (Research Center for Biotechnology, Indonesian Institute of Sciences)
Ade Andriani (Research Center for Biotechnology, Indonesian Institute of Sciences)
Filemon Jalu Nusantara Putra (Research Center for Biotechnology, Indonesian Institute of Sciences)
Erdiansyah Rezamela (Research Institute for Tea and Cinchona, Bandung)
Restu Wulansari (Research Institute for Tea and Cinchona, Bandung)
M Iqbal Prawira Atmaja (Research Institute for Tea and Cinchona, Bandung)
Puspita Lisdiyanti (Research Center for Biotechnology, Indonesian Institute of Sciences)



Article Info

Publish Date
05 Jan 2021

Abstract

Tea is one of the most popular beverages in the world. Produced from Camellia sinensis leaves, tea has been studied to provide health benefits due to the content of important metabolites. This study aimed to investigate the catechin contents, antioxidant, and antibacterial activities of Indonesian tea varieties, namely green tea, black tea, and white tea. Tea infusion was prepared by extracting 1 g of each sample into 10 mL of distilled water and incubated at 80 °C for 60 min. The catechin and epigallocatechin gallate (EGCG) contents of tea extracts were determined using high-performance liquid chromatography (HPLC). Antioxidant activity was measured using the free radical method with 2,2-diphenyl-1-pycrylhidrazyl (DPPH), while antimicrobial activity was assesed using paper disc diffusion assay. The results indicated that green tea had the highest contents of catechin (646 ± 17.14 mg/L) and EGCG (997.8 ± 36.72 mg/L), and antioxidant activity with IC50 of  5.65 µg/mL. Furthermore, green tea and white tea extracts showed inhibitory activity against Gram-positive bacteria such as Micrococcus luteus, Bacillus subtilis, and Staphylococcus aureus whereas black tea had no activity against all bacterial strains tested. Generally, we concluded that white tea and green tea contributed to the higher content of catechins and exhibited strong antioxidant and antibacterial properties.

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Journal Info

Abbrev

annales

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

Description

The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as ...