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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology ANNALES BOGORIENSES
Puspita Lisdiyanti
Research Center for Biotechnology, Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology Veterinary

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IDENTIFICATION OF ENDOPHYTIC BACTERIA FROM Curcuma zedoaria BASED ON PROTEIN PROFILE USING MALDI-TOF MASS SPECTROMETRY Tri Ratna Sulistiyani; Puspita Lisdiyanti
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 2 (2018)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2018.25.2.795

Abstract

Valid identification of microorganisms is a vital information to establish culture collections. Currently, molecular approach based on 16S rDNA is widely used for bacterial identification. This approach  is however, time consuming and expensive. Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) allows the identification of microorganisms directly from colonies and it only takes some few minutes. The interest of this study was to identify endophytic bacteria associated with Curcuma zedoaria based on protein profile using MALDI-TOF MS system and compare with 16S rDNA sequence results. Endophytic bacteria were isolated from part of medicinal plant C. zedoaria collected from Bogor, West Java Indonesia. The identification of selected bacteria was performed by protein profile using MALDI-TOF MS. A total of 66 endophytic bacteria from C. zedoaria plant, were selected for identification. The result of MALDI-TOF MS analysis showed that eleven isolates (16.67%) were correctly identified to the species level and 23 isolates (34.85%) matched on genus level of molecular approach. These results demonstrates that the MALDI-TOF system is suitable and feasible approach for the bacterial identification, mainly for screening and grouping of large number isolates.
Catechin Contents, Antioxidant and Antibacterial Activities of Different Types of Indonesian Tea (Camellia sinensis) Isa Nuryana; Shanti Ratnakomala; Fahrurrozi Fahrurrozi; Ario Betha Juanssilfero; Ade Andriani; Filemon Jalu Nusantara Putra; Erdiansyah Rezamela; Restu Wulansari; M Iqbal Prawira Atmaja; Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 24, No 2 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n2.106-113

Abstract

Tea is one of the most popular beverages in the world. Produced from Camellia sinensis leaves, tea has been studied to provide health benefits due to the content of important metabolites. This study aimed to investigate the catechin contents, antioxidant, and antibacterial activities of Indonesian tea varieties, namely green tea, black tea, and white tea. Tea infusion was prepared by extracting 1 g of each sample into 10 mL of distilled water and incubated at 80 °C for 60 min. The catechin and epigallocatechin gallate (EGCG) contents of tea extracts were determined using high-performance liquid chromatography (HPLC). Antioxidant activity was measured using the free radical method with 2,2-diphenyl-1-pycrylhidrazyl (DPPH), while antimicrobial activity was assesed using paper disc diffusion assay. The results indicated that green tea had the highest contents of catechin (646 ± 17.14 mg/L) and EGCG (997.8 ± 36.72 mg/L), and antioxidant activity with IC50 of  5.65 µg/mL. Furthermore, green tea and white tea extracts showed inhibitory activity against Gram-positive bacteria such as Micrococcus luteus, Bacillus subtilis, and Staphylococcus aureus whereas black tea had no activity against all bacterial strains tested. Generally, we concluded that white tea and green tea contributed to the higher content of catechins and exhibited strong antioxidant and antibacterial properties.
Co-Authors Ade Andriani Ario Betha Juanssilfero Erdiansyah Rezamela Fahrurrozi Fahrurrozi Filemon Jalu Nusantara Putra Isa Nuryana M Iqbal Prawira Atmaja Restu Wulansari Shanti Ratnakomala Tri Ratna Sulistiyani