First Molecular Identification of Sunfish in North Bali Water

I Made Oka Riawan (Ganesha Education University)
Gede Iwan Setiabudi (Ganesha Education University)
I Made Merdana (Udayana University)
I Putu Mangku Mariasa (True Scuba Bali)
Kadek Teguh Wirasastra (Nasional Maritim Sistem Bali)

Advances in Tropical Biodiversity and Environmental Sciences
Vol 3 No 1 (2019): ATBES

Stranded Sunfish in North Bali with full body we collect to do molecular identification. Samples were amplified at the d-loop locus (control region) using the PCR (Polymerase Chain Reaction) method. Primers used in PCR are H16498 as primary front (forward) and L15812 as reverse primer. Similarity value of 95% after alignment with Mola ramsayi (accession number accession AY940824) on GenBank, and the gaps of the nucleotide just 1%. The stranded sunfish identified using partial sequence mtDNA is the same species as the species Mola ramsayi.