FTA Cards are an easy way to collect blood samples, deliveries, and stores. The totalvolume of blood samples is less is needed when compared to the blood samples taken through a vein(venipuncture). The purpose of this research is a method that can be applied to isolate DNA samplesfrom FTA Cards using the Wizard® Genomic DNA Purifi cation Kit (Promega), PureLink® GenomicDNA Kits, and Chelex-100. This research was a type of experimental research, and data analysis wasshowed in qualitative and quantitative descriptively. The samples used were 16 samples of human bloodtaken using a lancet and then placed on the FTA Card, sampling was done by accidental samplingtechnique. The variations of the PureLink® Genomic DNA kits method with the addition of SDS 10%showed thin and smear DNA bands on agarose gels. Furthermore, the Chelex-100 method showed aclear DNA band. The purity data obtained varied between 1 to 1.1 and concentrations between 190 to950 ng/μl. The conclusion of this study is DNA kits method with the addition of 10% SDS and theChelex-100 method still have impurities, with the purity value of DNA was less than 1.8 and a lowconcentration to be used as a DNA template for further molecular analysis.
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