<![CDATA[Terpenoids screening were carried out using Liebermann Burchard and Salkowski reagent on the extract of Saputangan leaves. It showed that the leaves contained terpenoid compounds with appeared of a reddish brown ring in the extract and a reddish brown stain appeared on the TLC plate tested with 1% CeSO4 reagent in 10% H2SO4. The macerate of saputangan leaves processed separation using the partition method (Liquid-liquid Extraction). Extracts dissolved with methanol were partitioned with n-Hexane and then partitioned between aquadest and ethyl acetate in a ratio of 1: 1 to obtain 50 g of total terpenoids. Furthermore, TLC analysis was performed on total terpenoids using n-hexane: ethyl acetate (80:20 v/v) solvent to obtain 11 separate stains on the TLC plate with different Rf each. Analysis was enhanced in HPLC using 100% acetonitrile and 0.1% phosphoric acid at a wavelength of 210 nm, a flow rate of 0.500 mL/min and eluted for 30 minutes. Based on the HPLC results, there were 25 peaks which indicated the presence of total terpenoid compounds with the highest peak being peak no. 8 (ret.time's 6.234, area's 8503532 and height's 276032), peak no. 9 (ret.time's 6.674, area's 3322572 and height's 141859) and peak no. 10 (ret.time's 7.288, area's 2758231 and height's 103927)]]>
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