Polymerase Chain Reaction Technique: Overview and Evolution Uses

Arpian Herponi (Department of Obstetrics and Gynecology, Dr. Mohammad Hoesin General Hospital, Palembang, Indonesia)

Natural Sciences Engineering and Technology Journal
Vol. 1 No. 2 (2021): Natural Sciences Engineering and Technology Journal

Polymerase chain reaction (PCR) is a laboratory technique used to amplify or create multiple copies of a specific segment of DNA. This literature review aimed to describe the overview and uses of polymerase chain reaction techniques in biomolecular science. There are two principal regions of PCR usage in bioscience. They are high-throughput PCR frameworks and microfluidics-based PCR gadgets for purpose-of-care (POC) applications. In conclusion, the effective PCR enhancement of GC-rich DNA groupings addresses another test. It is confounded by the generation of auxiliary constructions impeding full denaturation and preliminary tempering and addresses numerous procedures to beat this issue that has been set up.