Aktivitas Antioksidan dan Anti-Elastase dari Ekstrak Etanol Tomat (Solanum lycopersicum L.)

Henderson, Alhoi Hendry (University Prima Indonesia)
Lister, I Nyoman Ehrich (Department of Physiology, Faculty of Medicine, University Prima Indonesia)
Fachrial, Edy (Laboratory of Biomolecular, Faculty of Medicine, University Prima Indonesia)
Girsang, Ermi (Department of Public Health, Faculty of Medicine, University Prima Indonesia)

Buletin Penelitian Tanaman Rempah dan Obat
Vol 31, No 2 (2020): Buletin Penelitian Tanaman Rempah dan Obat

Skin aging due to the damage caused by ultraviolet radiation and toxic ingredients in cosmetics is still a problem. Tomato has antioxidant and skin protection activities. The study aimed to investigate the potential of tomato as an antioxidant and elastase inhibitor. A 170 g of tomato simplicial powder was extracted using ethanol 70% by the maceration method. Antioxidant activity was measured through 2,2’-Azino-bis 3-ethyl benzothiazoline-6-sulphonic acid (ABTS)-reducing activity. The antiaging activity was measured through anti-elastase activity. Tomato extract (SLE) showed strong ABTS-reducing activity (IC50 = 86.66 ± 10.58) and very strong anti-elastase activity (IC50 = 19.73 ± 0.44). In conclusion, there was a linear correlation between antioxidant activity and anti-elastase activity. However, the antioxidant activity and anti-elastase activity of tomatoes were still below lycopene (IC50 antioxidant = 49.23 ± 2.06 µg.ml-1 and IC50 anti-elastase = 10.39 ± 0.43 49.23 ± 2.06 µg.ml-1). However, it was worth to be developed as a natural product as an antioxidant and anti-elastase.  Further study is required to do fractionation to get the purer lycopene compound from tomato.