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All Journal HAYATI Journal of Biosciences Jurnal Natur Indonesia JBIO: jurnal biosains (the journal of biosciences) Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung Pharmaciana: Jurnal Kefarmasian JURNAL ILMU PERTANIAN Buletin Penelitian Tanaman Rempah dan Obat Global Medical and Health Communication Biology, Medicine, & Natural Product Chemistry Majalah Obat Tradisional JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) Pharmascience The Indonesian Biomedical Journal Universa Medicina BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Penelitian Pendidikan IPA (JPPIPA) Preventif Journal Jambura Journal of Health Sciences and Research Journal Health & Science : Gorontalo Journal Health and Science Community Seminar Nasional Lahan Suboptimal Jurnal Kesehatan Journal of Health Sciences Jurnal Pendidikan dan Konseling PREPOTIF : Jurnal Kesehatan Masyarakat Buletin Penelitian Tanaman Rempah dan Obat International Journal of Basic and Applied Science JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan) Buletin Kedokteran dan Kesehatan Prima JURNAL KIMIA SAINTEK DAN PENDIDIKAN International Journal of Health and Pharmaceutical (IJHP) Journal of Social Science JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR) Jurnal Kedokteran STM (Sains dan Teknologi Medik) Ranah Research : Journal of Multidisciplinary Research and Development Jurnal Mahasiswa Ilmu Kesehatan Nutriture Journal
Edy Fachrial
Fakultas Kedokteran, Kedokteran Gigi Dan Ilmu Kesehatan, Universitas Prima Indonesia, Medan
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ISOLASI DAN AKTIVITAS ANTI MIKROBA BAKTERI ASAM LAKTAT DARI FERMENTASI NIRA KELAPA SAWIT Fachrial, Edy; Harmileni, Harmileni
BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Vol 5, No 1 (2018): Agustus
Publisher : Program Studi Biologi, Fakultas Biologi Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (939.827 KB) | DOI: 10.31289/biolink.v5i1.1707

Abstract

The aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method. Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and then incubated for 48 hours. The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6. The selected colonies are classified as Gram positive and bacilli in morphology. Antimicrobial activity against pathogenic bacteria was investigated using disc diffusion method. Antimicrobial activity of isolates against E.coli and S.aureus were 12,2 mm; 9mm; 11mm; 11mm; 10,5mm; 12 mm dan 13,5mm; 11mm; 12mm; 11,5mm; 12mm; 12,5mm respectively. from these result it can be concluded that LAB isolated from oil palm sap has potential as probiotic.
AKTIVITAS ANTIMIKROBA BAKTERI ASAM LAKTAT YANG DIISOLASI DARI MAKANAN TRADISIONAL FERMENTASI KHAS BATAK “NANIURA” Aloysius, Aloysius; Ulfa, Anjurniza; Situmorang, Anggita Kasih Fianti; Harmileni, Harmileni; Fachrial, Edy
BIOLINK : Jurnal Biologi Lingkungan, Industri dan Kesehatan Vol 6, No 1 (2019): Agustus
Publisher : Program Studi Biologi, Fakultas Biologi Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v6i1.2165

Abstract

Lactic acid bacteria (LAB) could be isolated from various fermented food products. One potential source of LAB is traditional fermented food. The aim of the study was to isolate and investigate antimicrobial activity of LAB isolated from traditional Batak food, “naniura”. The LAB isolates were characterized by Gram staining, fermentation type and catalase test. The investigation of antimicrobial activity of LAB against pathogenic bacteria were conducted using disc diffusion method. The results showed that 6 isolates of BAL were successfully isolated namely BN1, BN2, BN3, BN4, BN5 and BN6 had characteristics of Gram positive, rod shaped and catalase negative. All selected isolate have heterofermentation type. Four isolates (BN1, BN2, BN5 and BN6) were able to inhibit S. aureus, E. coli and S. typhi with inhibition zone diameters ranging from 6,9 to 12,3 cm. Based on the result, it was concluded that LAB isolated from naniura has potential as a source of probiotics.
Comparison of Antioxidant and Anti-hyaluronidase Potentials of Pineapple Core Extract (Ananas comosus (L.) Merr.) and Luteolin Jusri, Ridza; Widodo, Wahyu Setia; Widowati, Wahyu; Armansyah, Adek; Sormin, Delores Elisabeth; Fachrial, Edy; Lister, I Nyoman Ehrich
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n2.1629

Abstract

Skin aging processes are divided into chronological aging and premature aging. Premature aging is generally caused by free radicals, from both air pollution and photoaging. Natural compounds from plant extracts are among sources of antioxidants and anti-hyaluronidase which have the ability to prevent antiaging. One of the potential fruits related to antioxidant and antiaging activities is Anana scomosus. A. comosus has a number of phenolic compounds with biological activities. One of the main phenolic compounds in A. comosus is luteolin. The aim of this study was to evaluate the antioxidant and antiaging potentials of Pineapple Core Extract (PCE). This study was conducted at the Biomolecular and Biomedicine Research Center, Aretha Medika Utama from August to November 2018. Analysis of antioxidants from PCE and luteolin was carried out using H2O2 scavenging activity assay. The antiaging assay was carried out through inhibition of hyaluronidase enzyme, one of the important enzymes in the aging process. Luteolin had lower IC50 value of H2O2 scavenging activity of around 24.12±3.13 μg/ml, which was better than CPE with IC50 of 304.56±3.76μg/mL. The results of hyaluronidase inhibition activity assay showed that luteolin compound had a lower IC50 value of 67.38±3.99 μg/mL when compared to PCE with an IC50 value of 161.15±1.05 μg/mL. Hence, Luteolin has higher antioxidant and anti-hyaluronidase activities than PCEPerbandingan Aktivitas Antioksidan dan Antihialuronidase Ekstrak Bonggol Buah Nanas (EBN) dengan Senyawa LuteolinTerdapat dua jenis proses penuaan kulit, penuaan kronologis dan penuaan dini. Penuaan dini umumnya disebabkan oleh radikal bebas, baik dari polusi udara maupun photoaging. Penggunaan antioksidan dan antihialuronidase untuk mencegah proses penuaan telah banyak diketahui. Ananascomosus merupakan salah satu tanaman yang memiliki potensi sebagai antipenuaan dan antioksidan karena mengandung sejumlah senyawa fenolik yang memiliki berbagai aktivitas biologis. Salah satu senyawa fenolik utama dalam A. comosus adalah luteolin. Tujuan penelitian ini adalah untuk mengevaluasi potensi antioksidan dan anti-aging dari Ekstrak Bonggol Nanas (EBN) dan senyawa pembandingnya yaitu luteolin . Penelitian ini dilakukan di Biomolecular and Biomedicine Research Center, Aretha Medika Utama dari Agustus hingga November 2018. Pengujian antioksidan dari EBN dan luteolin  dilakukan menggunakan uji pemerangkapan H2O2. Uji antipenuaan dilakukan melalui uji penghambatan aktivitas hialuronidase, salah satu enzim penting dalam proses penuaan. Hasil penelitian menunjukkan bahwa Luteolin  memiliki nilai IC50 aktivitas pemerangkapan H2O2lebih rendah sekitar 24.12±3.13 μg/ml lebih baik daripada EBN dengan IC50 sebesar 304.56±3.76μg/mL. Hasil uji aktivitas penghambatan hialuronidase, senyawa luteolin  memiliki nilai IC50 lebih rendah, yaitu sebesar 67,38±3,99 μg/mL dibanding dengan EBN nilai IC50 161,15±1,05μg/mL. Hasil ini menunjukkan bahwa senyawa pembanding luteolin  memiliki aktivitas antioksidan dan antihialuronidase yang lebih tinggi daripada EBN. 
Antioxidant and Antityrosinase Activities of Ethanolic Pachyrhizuserosus Peel and Tuber Extract Siregar, Irma Dolsyeria; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Marpaung, Hans Hotma; Ferdinand, Sahna; Fachrial, Edy; Lister, I Nyoman Ehrich
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.515 KB) | DOI: 10.15395/mkb.v51n2.1628

Abstract

Aging process is a physiological process in living organisms caused by, among others, free radicals. One of the free-radical-related aging problems is skin hyperpigmentation (excessive melanin) due to increasing tyrosinase enzyme activities. Natural compounds are widely used as antioxidant and antiaging agents. Bengkuang (Pachyrhizuserosus) is known as a source of various active compounds which can be used against free radicals to reduce the risk of skin aging  through  tyrosinase enzyme inhibition. This study was performed in September 2018 in Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung, Indonesia to examine the antioxidant and antityrosinase properties of Pachyrhizuserosus peel extract (PPE) and Pachyrhizuserosus  tuber extract (PTE).The extraction of PPE and PTE was performed using 70% ethanol by maceration method, followed by  phytochemical analysis using modified Farnsworth method. Antioxidant activities were measured through 2,2-Diphenyl-1-picrylhydrazil (DPPH) scavenging activities while antiaging assay were conducted through the  tyrosinase activity inhibition. In this study, PPE contained saponin, tannin, triterpenoid, and terpenoid while the PTE showed the presence of flavonoid, saponin, phenol, tannin, and alkaloid in phytochemical analysis. In the antioxidant assay, PPE presented a higher DPPH scavenging activities (IC50= 84.09 µg/mL) when compared to PTE (IC50= 98.30 µg/mL)(p<0.05). In antiaging assay, PPE showed a higher tyrosinase inhibitory activities when compared to PTE with =97.05µg/mL and 194.51µg/mL,respectively. It can be concluded that PPE has antioxidant and antiaging activities effective for preventing skin aging. Aktivitas Antioksidan dan Antitirosinase Ekstrak Etanol Kulit dan Daging PachyrhizuserosusProses penuaan merupakan suatu proses fisiologis yang terjadi pada makhluk hidup yang dapat disebabkan oleh radikal bebas. Hiperpigmentasi kuli tmerupakan salah satu masalah penuaan yang disebabkan oleh radikal bebas melalui peningkatan aktivitas enzim tirosinase. Bengkuang (Pachyrhizus erosus) diketahui mengandung berbagai senyawa aktif yang dapat menangkal radikal bebas serta mengurangi risiko penuaan kulit. Penelitian dilaksanakan pada September 2018 di Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung, Indonesia untuk menguji kemampuan aktivitas antioksidan dan antitirosinase pada ekstrak etanol kulit bengkuang (EEKB) dan ekstrak etanol daging bengkuang (EEDB). Pembuatan ekstrak kulit dan daging bengkuang dilakukan dengan cara mengekstraksi bahan dengan etanol 70% menggunakan metode maserasi, kemudian dilanjutkan dengan analisis fitokimia ekstrak dengan modifikasi metode Farnsworth. Aktivitas antioksidan diuji dengan mengukur pemerangkapan 2,2-Diphenyl-1-picrylhydrazil (DPPH) sedangkan antiaging diuji dengan mengukur aktivitas penghambatan tirosinase. Pada uji fitokimia menunjukkan EEKB memiliki kandungan senyawa saponin, tanin, triterpenoid dan terpenoid, sedangkan EEDB menunjukkan kandungan senyawa flavonoid, saponin, fenol, tanin, dan alkaloid. Pada uji antioksidan, EEKB memiliki aktivitas tertinggi pada pemerangkapan DPPH (IC50=84.09 µg/mL) dibanding dengan EEDB (IC50=98.30 µg/mL) (p≤0.05). Pada pengujian antiaging, EEKB memiliki nilai yang lebih tinggi pada aktivitas penghambatan tirosinase dibandingkan dengan EEDB (IC50=97.05 µg/mL; 194.51 µg/mL (p≤0.05). Simpulan, EEKB memiliki aktivitas antioksidan dan antiaging sehingga efektif dalam mencegah penuaan kulit. 
Comparison of Antioxidant and Anti-Tyrosinase Activities of Pineapple (Ananas comosus) Core Extract and Luteolin Compound Vrianty, Dela; Qodariah, Rismawati Laila; Widowati, Wahyu; Sinaga, Ade Putra Fratama; Fibrina, Dewi; Fachrial, Edy; Lister, I Nyoman Ehrich
Jurnal Kedokteran Brawijaya Vol 30, No 4 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.679 KB) | DOI: 10.21776/ub.jkb.2019.030.04.2

Abstract

Free radicals and UV exposure can cause aging. Aging prevention needs substances that can prevent molecular oxidation reactions in cells and inhibit the activity of enzymes that trigger aging. Research on pineapple skin and flesh extract (Ananas comosus (L.) Merr.) reported the presence of luteolin compound which functions as antioxidants and antityrosinase. However, in this study, the object used was pineapple core extract (PCE), which has not been widely known for its antioxidant and antityrosinase activity. Therefore, the purpose of this study was to determine the content of phytochemical compounds, antioxidant activity, and inhibitory activity of tyrosinase enzymes by PCE and then compared with the luteolin (LT) compound test results using Fansworth method, DPPH scavenging activity assay and tyrosinase enzyme inhibition assay. Based on the study results, the phytochemical compounds contained in PCE were tannins and triterpenoids.  PCE and LT produced the highest DPPH scavenging activity, which was 64.86% and 59.32% (final concentration 200μg/ml and 6.25μg/ml) and the highest tyrosinase inhibition activity, which was 60.52% and 85.02% (final concentration 100 μg/ml). Antioxidant activity was determined based on IC50 of 87.46µg/mL and 4.17 µg/ml respectively. IC50 tyrosinase enzyme inhibition EBN and LT respectively at 62.27µg/ml and 5.25 µg/ml. Antioxidant activity through DPPH free radical scavenging test and tyrosinase enzyme inhibition activity by PCE was lower than LT.
Antioxidant and Anti-Hyaluronidase Activities of Dragon Fruit Peel Extract and Kaempferol-3-O-Rutinoside Liana, Liana; Rizal, Rizal; Widowati, Wahyu; Fioni, Fioni; Akbar, Khainir; Fachrial, Edy; Lister, I Nyoman Ehrich
Jurnal Kedokteran Brawijaya Vol 30, No 4 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.286 KB) | DOI: 10.21776/ub.jkb.2019.030.04.3

Abstract

Aging is a natural process in human life and is triggered by the presence of free radicals (ROS). The use of antioxidants from natural ingredients is one of the breakthroughs to overcome aging and counteract the harmful effects caused by the free radicals. This study aimed to determine and compare the antioxidant activity of H2O2 scavenging and hyaluronidase inhibition of red dragon fruit peel extract (DFPE) and kaempferol-3-o-rutinoside (KOR) compounds. Dragon fruit peel extract (DFPE) is obtained through extraction by maceration method using 70% ethanol solvent. The design of this study included antioxidant and anti-aging activity assay of EKBN and KOR at the series concentration of 15.63; 31.25; 62.50; 125; 250; 500 µg/mL through H2O2 scavenging, as well as the DFPE and KOR hyaluronidase inhibition assay at the series concentration of 5.21; 10.42; 20.83; 41.7; 83.33; 166.67 µg/mL. EKBN shows that the average activity of H2O2 scavenging is lower than KOR. In addition, the IC50 values of KOR for H2O2 scavenging is lower (351.46±2.30ug/mL) than DFPE (409.64±23.17ug/mL). While, KOR also has higher values of inhibitory activity than of the DFPE. However, the IC50 value of KOR for hyaluronidase inhibition activity was 84.07±10.46µg/mL, equivalent to the IC50 value of DFPE (85.32±10.24µg/mL). The presence of antioxidant and anti-aging activity in the EKBN is probably caused by betalain and the KOR compound itself contained in red dragon fruit. The results of the paired-samples T-test on antioxidant activity and anti-aging of DFPE and KOR showed non-significant difference. Thus, DFPE has an equivalent antioxidant and anti-aging through H2O2 scavenging and hyaluronidase activity as possessed by the KOR compound.
Isolation, Characterization and Antibacterial Activities of Lactic Acid Bacteria Isolated From Batak's Special Food "Dali Ni Horbo” Nasution, Muhammad Hasan Basri; Ramadhani, Shafira; Fachrial, Edy
Jurnal Natur Indonesia Vol 18, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.779 KB) | DOI: 10.31258/jnat.18.1.1-11

Abstract

Lactic Acid Bacteria (LAB) are gram-positive bacteria that are rod or round in shape, do not form spores, and do not have cytochromes. LAB can be isolated from fermented food products. LAB is useful to inhibit the growth of pathogenic bacteria so that they are potential antibacterial sources. The purpose of this study was to isolate LAB from the traditional food Dali Ni Horbo, continued with characterization, antimicrobial testing, and sensitivity testing towards several antibiotics. From the results of the study, the number of colonies obtained was 2.4 x 107 CFU/mL. 6 isolates were randomly selected, coded as isolates UPDH1, UPDH2, UPDH3, UPDH4, UPDH5, UPDH6, respectively. Biochemical characterization showed that the isolates were gram-positive and catalase-negative bacteria.  Results of antibiotic sensitivity testing using 6 antibiotics, namely amoxicillin (AML), erythromycin (E), oxacillin (OX), ofloxacin (OFX), cefotaxime (CTX), and gentamicin (CN), showed that all the isolates were resistant to CTX. The UPDH1 isolate was resistant to AML and OX. The UPDH3, UPDH5, and UPDH6 isolates were resistant to AML, and the UPDH4 isolate was resistant to E, OFX, CTX, CN, and OX. Antimicrobial tests used the disk diffusion method against Escherichia coli and Staphylococcus aureus. The UPDH2 isolate, gave an inhibition zone of 6.7 mm, and UPDH5 and UPDH6 gave inhibition zones of 7.6 mm and 8.5 mm respectively, against E. coli. Microbial inhibition tests against S. aureus by UPDH1, UPDH2, UPDH5 and UPDH6 gave inhibition zones of 13.5 mm, 9.0 mm, 12.1 mm and 12 mm respectively. From the results in this research it can be concluded that the traditional food Dali Ni Horbo is a potential source of probiotics.
AKTIVITAS INULINASE BAKTERI TERMOFILIK UTMSDA 6 YANG DIISOLASI DARI SUMBER AIR PANAS SIDEBUK DEBUK, SUMATERA UTARA Nabila, Pivi; Ainy, Al-Annisa Fadhila; Tarigan, Nuzulul Akbar; Fachrial, Edy; Lister, I Nyoman Ehrich
(Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) Vol 6, No 1 (2021): JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat)
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37887/jimkesmas.v6i1.16394

Abstract

Abstrak Indonesia masih membutuhkan impor untuk memenuhi kebutuhan enzim. Dibutuhkan 3 enzim untuk mengubahpati menjadi fruktosa dan menghasilkan 45% fruktosa. Enzim inulinase dinilai lebih baik karena membutuhkansatu tahap reaksi enzim dan menghasilkan 95% fruktosa. Bakteri termofilik dapat hidup hingga suhu 121°C dinilaimenjadi penghasil enzim termostabil yang efektif bagi pihak industri yang membutuhkan suhu tinggi. Tujuan daristudi ini mengisolasi dan karakterisasi bakteri termofilik penghasil enzim inulinase dari koleksi isolat lab biologimolekuler Universitas Prima Indonesia. Skrining inulinase menggunakan media Czapek Dox with 2% agar dan 1%inulin powder. Inkubasi selama 5-7 hari pada suhu 48-49°C. Hidrolisis inulin oleh koloni bakteri ditandai denganadanya zona bening setelah penuangan lugol iodine. Dari isolat yang diteliti dengan nama UTMSDA 6,karakterisasi secara biokimia menunjukan perubahan warna menjadi kuning-kuning, negatif pada tes hidrolisapati, tes keretakan media, tes sitrat, tes endapan hitam, serta tes hidolisa gelatin dan positif pada tes katalasedan tes motilitas. Tes morfologi menggunakan perwarnaan gram didapatkan batang gram-positif. Pengujianaktivitas enzim inulinase menggunakan spektrofotometer dengan panjang gelombang 570nm, didapatkaninkubasi jam ke-8 memiliki aktivitas inulinase paling tinggi sebesar 5,036 IU. Dari hasil penelitian, isolat memilikikemampuan sebagai sumber produksi inulinase termofilik yang berpotensi untuk digunakan di dunia industri.Keywords: Inulinase, bakteri termofilik, fruktosa, inulin
Aktivitas Antioksidan dan Anti-Elastase dari Ekstrak Etanol Tomat (Solanum lycopersicum L.) Henderson, Alhoi Hendry; Lister, I Nyoman Ehrich; Fachrial, Edy; Girsang, Ermi
Buletin Penelitian Tanaman Rempah dan Obat Vol 31, No 2 (2020): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v31n2.2020.67-74

Abstract

Skin aging due to the damage caused by ultraviolet radiation and toxic ingredients in cosmetics is still a problem. Tomato has antioxidant and skin protection activities. The study aimed to investigate the potential of tomato as an antioxidant and elastase inhibitor. A 170 g of tomato simplicial powder was extracted using ethanol 70% by the maceration method. Antioxidant activity was measured through 2,2’-Azino-bis 3-ethyl benzothiazoline-6-sulphonic acid (ABTS)-reducing activity. The antiaging activity was measured through anti-elastase activity. Tomato extract (SLE) showed strong ABTS-reducing activity (IC50 = 86.66 ± 10.58) and very strong anti-elastase activity (IC50 = 19.73 ± 0.44). In conclusion, there was a linear correlation between antioxidant activity and anti-elastase activity. However, the antioxidant activity and anti-elastase activity of tomatoes were still below lycopene (IC50 antioxidant = 49.23 ± 2.06 µg.ml-1 and IC50 anti-elastase = 10.39 ± 0.43 49.23 ± 2.06 µg.ml-1). However, it was worth to be developed as a natural product as an antioxidant and anti-elastase.  Further study is required to do fractionation to get the purer lycopene compound from tomato.
PENGARUH PENAMBAHAN PREBIOTIK INULIN TERHADAP AKTIVITAS ANTIBAKTERI LACTOBACILLUS CASEI Siregar, Hafiz Hawari; Silaban, Agnes Novica Putri; Sofiyana, Maria; Purba, Rosi Krisdayanti; Intisari, Yayo; Lister, I Nyoman Ehrich; Fachrial, Edy
Preventif Journal Vol 6, No 1 (2021): Preventif Journal
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37887/epj.v6i1.22594

Abstract

AbstrakInulin adalah salah satu prebiotik yang banyak dipergunakan sebagai sumber nutrisi bagi probiotik. Tujuan penelitian ini ialah untuk mengetahui efek penambahan prebiotik inulin pada aktivitas antibakteri Lactobacillus casei. Metode antibakteri dilakukan dengan metode difusi cakram. Dalam percobaan terpisah bakteri asam laktat yang telah diuji kemudian diinokulasi ke dalam tabung reaksi yang berisi MRS Broth 10 mL dan MRS Broth 10 mL yang telah ditambahkan dengan 5% tepung inulin. Kemudian diinkubasi dengan suhu 37 °C selama 24 jam.  Kemudian dilakukan kultur Bakteri patogen (Staphylococcus aureus) pada Nutrien Agar dengan cara mencelup kapas steril ke dalam tabung reaksi yang berisi bakteri patogen dan kemudian menyebarkannya secara merata pada Nutrien Agar steril dalam cawan petri. Nutrien Agar dalam cawan petri yang sudah diinokulasi pada suhu 37 °C selama 24 jam dengan bakteri patogen diinkubasikan. Hasil isolasi menunjukkan bahwa probiotik L. casei yang diisolasi dari minuman susu komersil memiliki aktivitas antibakteri spektrum luas. Probiotik L. casei ini efektif menghambat pertumbuhan bakteri S. aureus. Penambahan konsentrasi prebiotik inulin 0,5% (b/v) pada MRSB dapat menambah daya hambat bakteri Lactobacillus casei sebesar 8,4 mm terhadap pertumbuhan bakteri S.aureus. Untuk penelitan selanjutnya, perlu dilakukan pengamatan tentang berat molekul dan golongan bakteriosin yang dihasilkan oleh L. casei. Kata Kunci : Prebiotik, Inulin, Antibakteri, Lactobacillus casei
Co-Authors Abednego S. Karosekali Adek Amansyah Adek Armansyah Afif P Jati Ainy, Al-Annisa Fadhila Aisar Novita Alhoi Hendry Henderson Aloysius Aloysius Aloysius, Aloysius Angelin Christin Anggita Kasih Fianti Situmorang Anggraini, Sari Anjurniza Ulfa Annisa Amalia Ariatama, Bayu Arma Citra Armansyah, Adek Bayu Pratomo Bella Sari Sijabat Buter Samin Buter Samin Calvin Christian Karo Karo Chiuman, Linda Chrismis Novalinda Ginting Christine Halawa Clarissa Lister Cory Oriensia Siahaan DAMERIA DAMERIA Dea Christa Amanda Deborah Angelin Purba Delyuzar, Delyuzar Dini Ariyska Edward Kosasih Edwin Waroka Ehrich Lister, I Nyoman Eka Susilawati Erika Yolanda Ginting Ermi Girsang Ermi Girsang Ester Putri Mentary Mendrofa Felix Felix Ferdinand, Sahna Fibrina, Dewi Fioni, Fioni Ginting, Chrismis Novalinda Girsang, Ermi Girsang, Ermi Hakim Nasution, Ikhwanul Hanna Christsela Purba Hanna Sari Widya Kusuma, Hanna Sari Widya Harmileni Henderson, Alhoi Hendry Henny Safrita Ginting I Nyoman Ehrich Lister I Nyoman Ehrich Lister I Nyoman Ehrich Lister I Nyoman Ehrich Lister I Nyoman Ehrich Lister I Nyoman Ehrich Lister I Nyoman Ehrich Lister I Nyoman Enrich Lister I. Nyoman Ehrich Lister Indra Setiawan Rambe Intan Hasibuan, Febria Nurul Intisari, Yayo Ira Manda Sari Berampu Ismawati Ismawati ismawati Juli elisabeth mayen Juli Elisabeth Mayen Meliala Juliana Lina Jusri, Ridza Karo Sekali, Abednego Suranta Khainir Akbar, Khainir Komala, William Laura J. Pinem Lela Diyana Br Tarigan Liana Liana Liena Liena Linda Chiuman Lisma Viany Lister Ehrich I Nyoman Lister, Clarissa Lister, I Nyoman Ehrich Lister, I Nyoman Ehrich Lister, I Nyoman Ehrich Lister, Nyoman Ehrich Lokot Donna Lubis M. Andriady Saidi Nst Marbun, Togi Rut Marlita Marpaung, Hans Hotma Masdalena Nasution Muhammad Aditya Kurnia Nabila, Pivi Naidu, Ramawathy Nasution, Muhammad Hasan Basri Natalia Desvalentina Lase Neswita, Elfia Niranti, Tasya Putri Novriani, Erida Nyoman Ehrich Lister, I Obet Edom Ginting Ongko, Nicolas Xavier Pinem, Laura Juita Piska, Finna pratomo, bayu Purba, Rosi Krisdayanti Qodariah, Rismawati Laila Rama Riana Sitinjak Ramadhani, Shafira Ricky Kinarta Sembiring Rika Silvany Rita Kusuma Ritonga, Lestina Rizal Rizal Rizal Rizal Rr. Anisa Siwianti Handayani Rudi Chandra Sahna Ferdinand Ginting Saipul Sihotang Saragih, Ria Natalia Sari Anggraini Saryono Saryono Saryono Sianturi, Naomi Silaban, Agnes Novica Putri Silalahi, Elsa Debora Sinaga, Ade Putra Fratama Siregar, Hafiz Hawari Siregar, Irma Dolsyeria Situmorang, Anggita Kasih Fianti Sofiyana, Maria Sonya Bonifacia Mahadi Sormin, Delores Elisabeth Sumartin, Sonia Apriani Sunarti Sunarti Surya Dharma Tandiah Asan Tarigan, Nuzulul Akbar Tengku Muhammad Sahiril Anhar Tengku Rachmi Hidayani Titania T Nugroho Titania Tjandrawati Nugroho Tri Widyawati Ulfa, Anjurniza Urip Harahap Vicky Vrianty, Dela Wahyu Widowati Wahyu Widowati WAHYU WIDOWATI Waroka, Edward Widodo, Wahyu Setia Widya Yanti Sihotang Wijaya, Raysha Awlia Wilsen Wilsen Yasmin, Nabila Yonias Saleh Tria Zebua Yuliani Mardiati Lubis Yunita Dewani