Molecular Identification and Morphological Characterization of Ficus sp. (Moraceae) in Bogor Botanic Gardens

Muhammad Rifqi Hariri (Research Center for Plant Conservation and Botanic Gardens - Indonesian Institute of Sciences)
Peniwidiyanti Peniwidiyanti (Research Center for Plant Conservation and Botanic Gardens, Indonesian Institute of Sciences, Jln. Ir. H. Juanda 13, Bogor, West Java)
Arifin Surya Dwipa Irsyam (Herbarium Bandungense (FIPIA), School of Life Sciences and Technology (SITH), Bandung Insitute of Technology (ITB), Labtek VC Building, Jatinangor, Jawa Barat, Sumedang)
Rina Ratnasih Irwanto (School of Life Sciences and Technology (SITH), Bandung Insitute of Technology (ITB), Labtek XI Building, Jl. Ganeca No. 10, Bandung, West Jawa)
Irfan Martiansyah (Research Center for Plant Conservation and Botanic Gardens, Indonesian Institute of Sciences, Jln. Ir. H. Juanda 13, Bogor, West Java)
Kusnadi Kusnadi (Research Center for Plant Conservation and Botanic Gardens, Indonesian Institute of Sciences, Jln. Ir. H. Juanda 13, Bogor, West Java)
Eni Yuhaeni (Research Center for Plant Conservation and Botanic Gardens, Indonesian Institute of Sciences, Jln. Ir. H. Juanda 13, Bogor, West Java)

Jurnal Biodjati
Vol 6, No 1 (2021): May

Ficus spp. belongs to the tribe Ficeae in the Moraceae family. Many members of this genus have been collected and grown in Bogor Botanic Gardens. There are 519 living collections of Ficus conserved since 1817, and 13 of them have not been identified until the species level. This research aimed to identify the Ficus sp. originated from Kaur Selatan (Bengkulu) using morphological and molecular approaches. Morphological characterization and herbarium specimen observation have been carried out to identify the Ficus sp. The molecular approach was conducted through DNA barcoding using ITS primer. The molecular identification using ITS sequence showed that Ficus sp. is Ficus crassiramea with 99.87% similarity to the sequence in NCBI. Morphological observation through herbarium specimen showed that there are 9 vegetative characters specific to Ficus crassiramea.