Physicochemical Properties Of 131I-Rutin Under Acidic Labeling Condition As A Radiolabeled Compound For The Diagnosis Of Cancer

Maula Eka Sriyani (BATAN)
Maudy Shintia (UPI)
endah rosyidiah (BATAN)
Witri Nuraeni (BATAN)
Adinda Saraswati (UPI)
Eva Maria Widyasari (BATAN)

Jurnal Sains dan Teknologi Nuklir Indonesia (Indonesian Journal of Nuclear Science and Technology)
Vol 22, No 1 (2021): February 2021

Radiolabeled rutin (131I-rutin) has potential use in future diagnosis of cancer. The physicochemical characteristics of 131I-rutin are essential in regard to its distribution and accumulation in the body. Therefore, to ensure successful delivery of a labeled compound, it is necessary to evaluate its physicochemical properties. The labeling of 131I-rutin was accomplished using the oxidation method assisted by Chloramine-T under acidic conditions. Radiochemical purity value was measured by thin-layer chromatography (TLC-SG F254) using 100% methanol as the mobile phase. The electrical charge of 131I-rutin was determined by the paper electrophoresis. Moreover, the lipophilicity (P) was evaluated by the partition coefficient in the organic-water liquid. Plasma protein binding was determined in vitro by precipitation method using a 5% trichloroacetic acid (TCA) solution. The results showed that 131I-rutin has a neutral charge and a lipophilicity value (Log P) of 0.395 ± 0.203 (hydrophilic), and was bound to human blood plasma proteins with a percentage of 69.36% ± 1.88%.