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All Journal Jurnal Sains dan Teknologi Nuklir Indonesia (Indonesian Journal of Nuclear Science and Technology)
Eva Maria Widyasari
BATAN
Biochemistry, Genetics & Molecular Biology Energy Environmental Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics

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PREPARASI SENYAWA ANTI KANKER APIGENIN BERTANDA RADIOIODIUM-131 UNTUK STUDI BIOAKTIVITAS Teguh Hafiz Ambar Wibawa; Eva Maria Widyasari; Iswahyudi Iswahyudi; Ade Suherman; Maula Eka Sriyani; Ahmad Kurniawan; Danni Ramdhani
Jurnal Sains dan Teknologi Nuklir Indonesia (Indonesian Journal of Nuclear Science and Technology) Vol 21, No 1 (2020): Februari 2020
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.12 KB) | DOI: 10.17146/jstni.2020.21.1.5788

Abstract

PREPARASI SENYAWA ANTI KANKER APIGENIN BERTANDA RADIOIODIUM-131 UNTUK STUDI BIOAKTIVITAS. Apigenin merupakan senyawa flavonoid yang mempunyai potensi sebagai senyawa anti kanker, anti oksidan, dan anti inflamasi dengan toksisitas intrinsik yang rendah. Untuk mengetahui bioaktivitas apigenin, dapat dilakukan radioiodinasi kemudian dilanjutkan dengan studi praklinis menggunakan hewan uji. Pada penelitian ini dilakukan preparasi apigenin bertanda radioiodium-131 menggunakan metode kloramin-T dan optimasi berbagai parameter serta kondisi penandaan. Hasil optimasi diperoleh formula dan kondisi penandaan yaitu 1 mg apigenin, 250 µg kloramin-T, dan 100 µg natrium metabisulfit, dengan pH penandaan 7 dan jumlah radioiodium-131 10 µL (10 µCi). Proses penandaan senyawa 131I-Apigenin dilakukan melalui reaksi substitusi elektrofilik selama 20 menit pada suhu kamar (20-22 °C). Dari hasil pengujian menggunakan metode kromatografi kertas menggunakan fasa diam Whatman 1 dan fasa gerak amonium asetat 0,02 M, pH 6, diperoleh kemurnian radiokimia sebesar 96,53 ± 1,87%. Hasil tersebut menunjukkan bahwa 131I-Apigenin dapat digunakan untuk studi selanjutnya, yaitu studi fisikokimia dan studi praklinis, sehingga dapat diperoleh karakteristik bioaktivitas dan efektivitasnya sebagai senyawa anti kanker berbasis bahan alam.
Physicochemical Properties Of 131I-Rutin Under Acidic Labeling Condition As A Radiolabeled Compound For The Diagnosis Of Cancer Maula Eka Sriyani; Maudy Shintia; endah rosyidiah; Witri Nuraeni; Adinda Saraswati; Eva Maria Widyasari
Jurnal Sains dan Teknologi Nuklir Indonesia (Indonesian Journal of Nuclear Science and Technology) Vol 22, No 1 (2021): February 2021
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jstni.2021.22.1.6286

Abstract

Radiolabeled rutin (131I-rutin) has potential use in future diagnosis of cancer. The physicochemical characteristics of 131I-rutin are essential in regard to its distribution and accumulation in the body. Therefore, to ensure successful delivery of a labeled compound, it is necessary to evaluate its physicochemical properties. The labeling of 131I-rutin was accomplished using the oxidation method assisted by Chloramine-T under acidic conditions. Radiochemical purity value was measured by thin-layer chromatography (TLC-SG F254) using 100% methanol as the mobile phase. The electrical charge of 131I-rutin was determined by the paper electrophoresis. Moreover, the lipophilicity (P) was evaluated by the partition coefficient in the organic-water liquid. Plasma protein binding was determined in vitro by precipitation method using a 5% trichloroacetic acid (TCA) solution. The results showed that 131I-rutin has a neutral charge and a lipophilicity value (Log P) of 0.395 ± 0.203 (hydrophilic), and was bound to human blood plasma proteins with a percentage of 69.36% ± 1.88%.
Co-Authors Ade Suherman Adinda Saraswati Ahmad Kurniawan Danni Ramdhani endah rosyidiah Iswahyudi Iswahyudi Maudy Shintia Maula Eka Sriyani Teguh Hafiz Ambar Wibawa Witri Nuraeni