Berkala Penelitian Hayati
Vol 21 No 1 (2015): December 2015

SCREENING OF RAPD PRIMER FOR TEAK (Tectona grandis)

Imas Cintamulya (University of PGRI Ronggolawe Jl. Manunggal 61 Tuban, East Java)



Article Info

Publish Date
27 Apr 2016

Abstract

Identification of DNA polymorphisms in teak is important. It is a first step to determine the presence of genetic varia-tion in teak. The information of genetic variation is needed for teak breeding development. RAPD is one of method which can be used for identification of DNA polymorphism. This study aim to get the RAPD primer which can detect the DNA polymorphism in teak. Benefits of this study are provide information about primer which can detect the DNA polymorphism in teak, DNA polymorphism data can be used for genetic variation analysis which needed for teak breeding development. The primers which used in this study shown the DNA polymorphism in teak. The primer are OPF6 (5'-GGGAATTCGG-3 '), OPF8 (5'-GGGATATCGC-3'), and OPF11 (5'-TTGGTACCCC-3 '). The highest DNA polymorphism is shown in DNA which amplified with OPF-8 primer.

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Journal Info

Abbrev

jurnal

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Description

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by ...