Berkala Penelitian Hayati
Vol 25 No 2 (2020): June 2020

The UV-Vis spectrum of antioxidant complex from frozen-dried egg white protein and tomato extract

Siti Imama Khoiriyah (Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jalan Veteran, Malang, East Java 65145, Indonesia)
Mohammad Mabrur (Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jalan Veteran, Malang, East Java 65145, Indonesia)
Sutiman Bambang Sumitro (Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jalan Veteran, Malang, East Java 65145, Indonesia)
Sri Widyarti (Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jalan Veteran, Malang, East Java 65145, Indonesia)



Article Info

Publish Date
20 Jun 2020

Abstract

In this study, we want to characterize egg white protein-tomato extract complex based on the UV-Vis spectrum. The formulation of antioxidant complex Non-Freeze-drying (NFD) was made at a ratio of 1:10 (v/v) while the Freeze-drying (FD) sample was made at a ratio of 1:2 (v/v). The results showed that the absorption peak of FD complex profile value was lower than the single ingredient, while the tomato extract was higher than the complex profile. On the contrary, the NFD complex showed a higher peak absorption value than to every single ingredient. Based on each UV-Vis spectrum profile, the tomato extract profile shifted the absorption peak, and the absorbance value of the FD sample was changing higher than the NFD sample, while the egg white protein spectrum profile did not change significantly in the FD and NFD samples. The absorption peak of the complex is higher than the single ingredient indicated that the characterization of the complex had been formed. In the NFD complex profile, the absorption peaks formed at the wavelength regions of 230 nm and 280 nm with absorbance values of 2.51 and 0.76 and its number has higher than those of the FD complex with absorbance values of 2.47 and 1.82 at wavelengths of 220 and 265 nm.

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Journal Info

Abbrev

jurnal

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Description

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by ...