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All Journal HAYATI Journal of Biosciences Media Veteriner Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Journal of Education and Learning (EduLearn) Research Journal of Life Science AGRIVITA, Journal of Agricultural Science Journal of Mathematical and Fundamental Sciences Molecular and Cellular Biomedical Sciences (MCBS) Biosfer: Jurnal Pendidikan Biologi Biosaintifika: Journal of Biology & Biology Education Jurnal Eduscience (JES) Berkala Penelitian Hayati Journal of Global Pharma Technology Jurnal Kedokteran Hewan Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education)

Sutiman Bambang Sumitro

Department Of Biology, Faculty Of Mathematics And Natural Sciences, Brawijaya University

Author-ID : 2515745

Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Social Sciences Veterinary Other

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MECHANISM OF INFECTION Spodoptera litura Nucleopolyhedrosis Multiple Virus (SpltMNPV) ON MIDGUT EPITHELIAL CELL ARMY WORM (Spodoptera litura) Observed by TEM Asri, Mahanani Tri; Chaelani, Siti Rasminah; Rahardjo, Bambang Tri; Sumitro, Sutiman Bambang
AGRIVITA, Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Spodoptera litura is one of Â agricultural crop pests. They are resistant to chemical insecticides. One of arternate biological control is Spodoptera litura Nucleopolyhedrosis Multiple Virus (SpltMNPV) Penelitian ini dilakukan untuk mengetahui bagaimana mekanisme SpLtMNPV dalam menginfeksi sel epithel midgut larva S. The research was conducted to determine how SpltMNPV infected midgut epithelial cells of S. litura secara in vitro . litura larvae in vitro.Â The mechanism of infection was observed by a transmission electron microscopy (TEM). Â The result of this observation showed that infecting midgut cell by Â SpltMNPV involved 5 phases, they are, 1) the attachment ofÂ SpltMNPV at the membrane of suitable host, 2) the penetration, formation of channels and release of protein envelope, 3) the biosynthesis of virus components in the cell nucleus, 4) the assembling of virus components, and 5) the releasing of MNPV/multiplenucleocapsid through budding. Keywords: mechanisms of infection, Spodoptera litura multiple nucleopolyhedrosis virus, midgut army worm larvae cells.

Isolation and Identification of Transforming Growth Factor β from In Vitro Matured Cumulus Oocyte Complexes . WIDJIATI; ARIEF BOEDIONO; SUTIMAN BAMBANG SUMITRO; AUCKY HINTING; . AULANI’AM; TRINIL SUSILOWATI
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

Transforming growth factor-β (TGF-β) is a two-chain polypeptide with molecular weight of 25 kDa which takes significant role in the steroidogenesis process. In the ovarian oocyte in particular, TGF-β has an important role in regulating reproductive function. TGF-β represents a key intrafollicular protein that regulates follicle development and aromatization process. The purpose of this research was to characterize and identify a protein fraction of TGF-β from the bovine isolated oocytes, which is synthesized during in vitro oocyte maturation process. Oocytes were collected from follicles with diameter of 3-8 mm. Oocytes were then matured in TCM 199 media supplemented with 5 μg/mg LH, 3% BSA, and 50 μg/ml gentamicin sulfate, and cultured in CO2 incubator (5%, 38.5 oC) for 20 hours. TGF-β receptors were identified immunohistochemically. Characteristics of the TGF-β protein were determined using SDS PAGE and TGF-β specification was tested using Western Blotting. The results showed that TGF-β receptors were identified and found in cumulus oocyte complexes (COCs). TGF-β protein was isolated from bovine oocytes with molecular weight 25 kDa and it was identified by Western blotting methods in the same molecular weight.

Developing complexity science-problem based learning model to enhance conceptual mastery Finga Fitri Amanda; Sutiman Bambang Sumitro; Sri Rahayu Lestari; Ibrohim Ibrohim
Journal of Education and Learning (EduLearn) Vol 16, No 1: February 2022
Publisher : Intelektual Pustaka Media Utama

Implementing a proper learning model during the post COVID-19 pandemic is fundamental for learning quality enhancement, specifically for students' conceptual mastery. The research aims to develop a Complexity Science- Problem Based Learning (CS-PBL) model assisted by the Sistem Informasi Pengelola Pembelajaran (SIPEJAR) e-learning platform that is valid, practical, and effective to enhance students' conceptual mastery during the post COVID-19 pandemic. The research and development model were adapted from Plomp and Nieven consisted of three phases: preliminary research, prototyping phase, and assessment phase. The first phase research result was that the learning process in during COVID-19 pandemic was less interactive, which led to less effective learning. The students' score on conceptual mastery was in the poor category. The second phase resulted in a book of CS-PBL model assisted by SIPEJAR and supporting instruments considered valid by three experts. The third phase result was that the CS- PBL model assisted by SIPEJAR was considered practical in the learning process implementation. The CS-PBL model can enhance students' conceptual mastery where the N-gain was sufficiently effective. It is concluded that the CS-PBL model assisted by SIPEJAR was considered valid, practical, and effective to enhance students' conceptual mastery during the post COVID-19 pandemic.

Percobaan ini dilaksanakan di Laboratorium Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya Malang dan Balai Inseminasi Buatan Singosari pada bulan Agustus sampai Desember 1998. Tujuan penelitian ini adalah untuk mencari metode pembekuan spermatozoa hasil seleksi jenis kelamin dengan menggunakan filtrasi sephadex G-200. Percobaan ini terdiri atas 10 kali ulangan dengan dua perlakuan yaitu krioprotektan ekstraseluler TCM- 199+ 10% serum+ 14% kuning telur dan tris aminom Trinil Susilawati; Sutiman Bambang Sumitro; soehartojo Hardjopranjoto; Mochammad Sasmito Djati; Gato Gato
Media Veteriner Vol. 6 No. 4 (1999): Media Veteriner
Publisher : Media Veteriner

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Percobaan ini dilaksanakan di Laboratorium Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya Malang dan Balai Inseminasi Buatan Singosari pada bulan Agustus sampai Desember 1998. Tujuan penelitian ini adalah untuk mencari metode pembekuan spermatozoa hasil seleksi jenis kelamin dengan menggunakan filtrasi sephadex G-200. Percobaan ini terdiri atas 10 kali ulangan dengan dua perlakuan yaitu krioprotektan ekstraseluler TCM- 199+ 10% serum+ 14% kuning telur dan tris aminomethan- kuning telur. Parameter yang diukur meliputi persentase motilitas, hidup, kapasitasi dan reaksi akrosom. Media TCM-199 kuning telur lebih dapat mempertahankan mutu semen dibandingkan dengan tris aminomethan kuning telur, dan proses pembekuan meningkatkan kapasitasi dan reaksi akrosom.

The Linnaeus’s Reed Snake, Calamaria linnaei Boie (Squamata: Colubridae: Calamariinae) from Ijen Plateau, East Java, Indonesia Irvan Sidik; Sutiman Bambang Sumitro; Nia Kurniawan
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The decisive morphological character of Calamaria linnaei from the Ijen plateau was analyzed in an attempt to evaluate these snakes independently achieved as different populations or whether more likely only cryptic species affected by natural conditions. The evidence was concluded that a cryptic species is very interesting to be regarded as something entirely different. In general, however, the population appears to have more closely related to other congeners populations of the same species. It is suggested that at least temporarily, the C. linnaei population of the Ijen plateau be specifically considered to include in species with high individual variations based on limited geographic boundaries until molecular analysis proves it.

Effects of Conditioned Medium of Co-Culture IL-2 Induced NK Cells and Human Whartonâs Jelly Mesenchymal Stem Cells (hWJMSCs) on Apoptotic Gene Expression in a Breast Cancer Cell Line (MCF-7) Wahyu Widowati; Diana Krisanti Jasaputra; Philips Onggowidjaja; Sutiman Bambang Sumitro; Mochammad Aris Widodo; Ervi Afifah; Dwi Davidson Rihibiha; Rizal Rizal; Annisa Amalia; Hanna Sari Widya Kusuma; Harry Murti; Indra Bachtiar
Journal of Mathematical and Fundamental Sciences Vol. 51 No. 3 (2019)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2019.51.3.1

Breast cancer (BC) is the most prevalent type of cancer among women and one of the major causes of cancer mortality in women. Metastasis in breast cancer (BC) occurs due to immunosurveillance deficiency, including impairment of natural killer (NK) cell maturation. Conditioned medium (CM) from human Wharton's jelly mesenchymal stem cells (hWJMSC-CM) is known to possess anticancer activity. The CM of co-culture of human recombinant IL-2 treated NK cells and hWJMSCs is expected to boost anticancer activity toward BC cells which can be analyzed from the effect of CM towards secretion of effector molecules and expression of BC cell apoptosis-related genes, and cytotoxic granules in human recombinant IL-2 treated NK (IL-2 NK) and hWJMSCs (IL-2 hWJMSCs). TNF-Î±, IFN-Î³, perforin, granzyme were measured by ELISA, while the inhibition of cell proliferation was measured by MTS assay and BC cell apoptosis by flow cytometry and apoptotic gene expression by RTPCR. CM from co-cultured hWJMSCs and IL-2 NK cells inhibited NK and BC cell proliferation, increased expression of Bax and p53 and decreased the number of Bcl-2 in BC cells. In conclusion, CM of co-culture IL-2 treated NK cells and hWJMSCs induce apoptosis in BC cells as indicated by increased Bax and p53 expression and decreased Bcl-2 expression.

Quercetin, rutin, proanthocyanidin, catechin and epitacethin as fatty acid synthase inhibitor using virtual screening Candra Rini Hasanah Putri; Sutiman Bambang Sumitro; Setyawati Karyono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

Fatty acid synthase is known to participate in the occurrence of malignancies, so fatty acid synthase inhibition is expected can restrain malignancy. In this research, virtual screening is done by molecular docking between the active ingredients in Tamarindus indica with three domains of FAS. In thioesterase domain, it turns out all of the active ingredients in Tamarindus indica can bind thioesterase domain right in the place where orlistat (as a reference inhibitor) bonded, with a higher strength than the orlistat. In enoyl [acyl-carrier-protein] reductase domain, it turns out the binding affinity of quercetin, rutin, catechin and epicatechin against fatty acid synthase are greater than the reference inhibitor, triclosan. In malonyl-CoA / acetyl-CoA-ACP-transacylase domain, it turns out the binding affinity of quercetin, rutine, proanthocyanidin, and catechin against fatty acid synthase is greater than the natural substrate, Malonyl-CoA. The high binding affinity of the active ingredients in Tamarindus indica against the two domains of fatty acid synthase that may also can be occupied by reference inhibitors, showed the ability of Tamarindus indica as an inhibitor of fatty acid synthase. While the high ability of active ingredients in Tamarindus indica to bind to a domain that should be occupied by the natural substrate of fatty acid synthase (malonyl-CoA) demonstrated the ability of Tamarindus indica to inhibit fatty acid synthase's work in a way to compete with the natural substrate. This study shows that Tamarindus indica may serve as anti-malignancy through its ability to inhibit fatty acid synthase.

The UV-Vis spectrum of antioxidant complex from frozen-dried egg white protein and tomato extract Siti Imama Khoiriyah; Mohammad Mabrur; Sutiman Bambang Sumitro; Sri Widyarti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

In this study, we want to characterize egg white protein-tomato extract complex based on the UV-Vis spectrum. The formulation of antioxidant complex Non-Freeze-drying (NFD) was made at a ratio of 1:10 (v/v) while the Freeze-drying (FD) sample was made at a ratio of 1:2 (v/v). The results showed that the absorption peak of FD complex profile value was lower than the single ingredient, while the tomato extract was higher than the complex profile. On the contrary, the NFD complex showed a higher peak absorption value than to every single ingredient. Based on each UV-Vis spectrum profile, the tomato extract profile shifted the absorption peak, and the absorbance value of the FD sample was changing higher than the NFD sample, while the egg white protein spectrum profile did not change significantly in the FD and NFD samples. The absorption peak of the complex is higher than the single ingredient indicated that the characterization of the complex had been formed. In the NFD complex profile, the absorption peaks formed at the wavelength regions of 230 nm and 280 nm with absorbance values of 2.51 and 0.76 and its number has higher than those of the FD complex with absorbance values of 2.47 and 1.82 at wavelengths of 220 and 265 nm.

HUMAN SPERM PROTEIN 116 KDA: A CANDIDATE ANTIGEN FOR IMMUNOCONTRACEPTION TECHNOLOGY Umi Lestari; Aulanni’am Aulanni’am; Basuki B. Purnomo; Sutiman Bambang Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Spermatozoa membrane antigenic protein utilized as the substance candidate of immunocontraception has important criteria, that are only expressed in spermatozoa, cannot be found in the other tissue, and also should not have kinase activities. The observations done finally conclude 116kD, a sperm head’s protein is within the above criteria. It’s antigenic characters are shown with ability of it’s polyclonal antibody to bind the human spermatozoa, and interestingly this polyclonal antibody enable to block fertilization of other species in an in-vitro test experiments. This reseach also acquired that 116 kDa protein is specific and exists only in spermatozoa, and not in the other tissue such as spleen, kidney, pancreas, and epidydimic. This 116 kDa protein distribute along the whole area of the sperm, but mostly accumulated around the head up to the the neck. The imunohistological staining of the testis also indicate that this protein exists in the spermatid of the testes, but not be found in other somatic tissues, and it’s antibody is evidently was recognised by the goat and cow spermatozoa membrane protein resulted in blocking the fertilization of both species respectively. And the conclussion, 116 kD is Non-kinase protein specific only exists in the area of the head of human spermatozoa. It is therefore believed to be adequate candidate for antigen substance of immunocontraception technology.

SELF ASSEMBLY AND MAGNETISM OF LIVING BIOLOGICAL MOLECULES Sutiman Bambang Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

Biological molecules are essentially nano size structure. All of them are complex structure with specifi c function dedicated to perform normal ordered organizational system. The forces for their work are non-covalent interactions; include spontaneous folding of proteins, DNA, RNA and other bio-macromolecules, ligand-receptors interactions, assembly-disassembly of macromolecule, andtransportation or movement of many other nano size sub cellular components. The non-covalent interactions are weak bonds system that is low energetic chemical and physical forces. The energetic forces are mainly atomic forces such as electromagnetic force emergence from electron spinning and transitions at every atom of the complex macromolecular structure. The energy will work along with different level of energy, and atomic positioning within macromolecules. This paper review and discuss the role of magnetism on molecular working process as part of thermodynamically open systems to develop order, which is constantly receiving, transforming and dissipating energy, can and do continually exhibit self assembly and organization, along with the self repairing, and perpetuation.

Co-Authors . AULANI’AM Agus Muji Santoso Ahmad Faried Annisa Amalia Arief Boediono Aucky Hinting Aulanni'am Aulanni'am Aulanni'am Aulanni'am Aulanni’am Aulanni’am Bambang Tri Rahardjo Bambang Tri Rahardjo Basuki B. Purnomo Bayu Guruh Binangkit Bayyinatul Muchtaromah Betty Lukiati Candra Rini Hasanah Putri Chomsa Dintasari Umi Baszary Diana Krisanti Jasaputra Dody Novrial, Dody Dwi Davidson Rihibiha Dwi Davidson Rihibiha Edi Widjajanto Ervi Afifah Ervi Afifah Fatchur Rohman Finga Fitri Amanda Finga Fitri Amanda* Gato Gato Gatot Ciptadi Hanna Sari Widya Kusuma Hanna Sari Widya Kusuma Harry Murti Harry Murti Ibrohim Ida Rahmawati Ignatius Riwanto Indra Bachtiar Indra Bachtiar Indra Wijaya Irvan Sidik Iwan, Iwan Karyono Mintaroem Mahanani Tri Asri Mahanani Tri Asri Maris Kurniawati Moch Sasmito Djati Mochammad Aris Widodo Mochammad Aris Widodo Mohamad Amin Mohammad Mabrur Mumun Nurmilawati Nia Kurniawan Nurul Jadid Mubarokati Philips Onggowidjaja Poppy Rahmatika Primandiri Rizal Rizal Rizal Rizal Setyawati Karyono Setyawati Soeharto Siti Imama Khoiriyah Siti Rasminah Chaelani soehartojo Hardjopranjoto Soemarno Soemarno Sri Rahayu Lestari Sri Wahjuningsih Sri Widyarti Suhartojo Hardjopranjoto Sulistiono TRINIL SUSILOWATI Umi Lestari Wahyu Widowati Wahyu Widowati Widjiati w Yayoi Kodama

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