KARAKTERISASI EKSTRAK KASAR FITASE TERMOFILIK DARI BAKTERI KAWAH IJEN BANYUWANGI, ISOLAT AP-17

Aline Puspita Kusumadjaja (Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Surabaya)
Tutuk Budiati (Fakultas Farmasi Universitas Airlangga Surabaya)
Sajidan (Jurusan Pendidikan Matematika dan Ilmu Pengetahuan Alam Fakultas Keguruan dan Ilmu Pendidikan Universitas Negeri Sebelas Maret)
Ni Nyoman Tri Puspaningsih (Jurusan Kimia Fakultas Sain dan Teknologi Universitas Airlangga Surabaya)

Berkala Penelitian Hayati
Vol 16 No 1 (2010): December 2010

Crude thermophilic phytase was produced by isolate AP-17 that has been isolated from Ijen Crater Banyuwangi. Based on Gram test, isolate AP-17 was gram positive spore forming rod shape bacteria so that it was identified as Bacillus sp. AP-17. Crude thermophilic phytase isolated from Bacillus sp. AP-17 had the optimum temperature at 75° C with activity of 0.1413 U/ml, and its optimum pH was at pH 6 with activity of 0.0875 U/ml. The enzyme was stable when heated at 75° C for three hours and still had 90% activity when it was exposed at pH 5–8, optimum temperature, for one hour.