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Review: Phylogenetic Similarity based on amino acid sequence and Molecular characterization of 3-Phytase from Klebsiella pneumonia ASR1 ., Sajidan; Hailu, Hailu Weldekiros
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Phytase is an enzyme that catalyzes the release of phosphomonester group in phytate, thereby producing lower forms of myo-inositol phosphates and inorganic phosphate.  Phytase has an important role in animal nutrition which improves the bioavailability of nutrients.  Bacteria are one of the potential sources of phytase. For this reason, more screening of phytase producing bacteria strains from environment is needed. The other sources of phytase are fungi, plants, and some animal tissues. The PhyK protein is a phytase enzyme from Klebsiella pneumonia ASR1 and expressed in Escherichia coli, which was sequenced and characterized as a 42 kDa [1], and Crystallized [2]. The objectives are to perform phylogenetic similarity and molecular evolutionary analysis of phytase and to determine active site protein motif of phytase from different sources. The sequence from PhyK was analyzed with Basic Local Alignment Search Tools (BLAST) at http://www.ncbi.nlm.nih.gov  and ClustalW Multiple Sequence analysis program (EMBL-EBI) at http://www.ebi.ac.uk/Tools/msa/clustalw2/ to get similarity of amino acid sequence and phylogenetic trees for protein.  The result shows that PhyK protein from Klebsiella pneumonia ASR1 is 91% similar with PhyK from Raoultella terrigena (emb|AJ575300.1|), 94% similar with histidine acid phosphatase family protein from Klebsiella pneumoniae 342, and 69% similar with glucose-1-phosphatase precursor Agp from Pantoea ananatis AJ13355 (BAK10365.1).The deduced amino acid sequence of the phyK gene from Klebsiella pneumonia ASR1, although containing the functional residues of histidine acid phosphatases, displayed only 31% overall homology with appA from Escherichia coli O104:H4 str. C227-11 (EGT68141.1), 30% similar with glucose-1-phosphatase from Pantoea agglomerans (gb| ABD85282.1|) and 26% to phytase from Aspergillus fumigatus (AAU93517.1). This suggests that PhyK represents a novel subfamily histidine acid phytate-degrading enzyme and is clearly distinct from other previously characterized members of this family. Keywords: Bioinformatics, BLAST, 3-Phytase, PhyK protein, Phylogenetic Similarity
PROPORTION: CLAIM, REBUTTAL AND BACKING DATA BASED ON TEACHER QUESTIONS AS REASONING INDICATOR OF PROBLEM BASED LEARNING IN HIGHSCHOOL SYSTEM REPRODUCTIONS Widoretno, S.; Dwiastuti, S.; Sajidan, S.
Jurnal Pendidikan IPA Indonesia Vol 5, No 2 (2016): October 2016
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v5i2.7182

Abstract

The reseach purpose is to compare learning proportion of the students based on arguments to learners Claim which is supported by facts, data and theories between conventional learning and PBL as the answers for teacher questions. Research Procedure began with 4 times biology teachers discussion workshops to compile PBL learning plan to be used on treatment class. 61 participants with 30 in control class and 31 in treatment class. Reasoning calculation based on arguments during communication in form of the answers to teacher questions. Basic Grouping of Learning is : Claim Rebuttal (CR), Claim Data (CD), Claim Backing (CB) and Claim (C). Results indicates that: C decreases during PBL learning; CB increases during PBL; CD  doesn’t exhibit real change during PBL. PBL utilization needs the readiness and competence of the teachers which influences the reasoning quality of the learners.
SKILL OF TEACHER CANDIDATES IN INTEGRATING THE CONCEPT OF SCIENCE WITH LOCAL WISDOM -, Parmin; Sajidan, S.; Ashadi, A.; Sutikno, S.
Jurnal Pendidikan IPA Indonesia Vol 4, No 2 (2015): October 2015
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v4i2.4179

Abstract

Learning science is not limited to reviewing the concepts, but strengthens the identity of a nation that has a diversity of cultures. Science learning objectives that have been set in Indonesia, including the student is able to apply the science wisely, to maintain and preserve the cultural survival. The study aims to measure students ability to relate concepts of science with local knowledge to use mind maps compiled individually. The results showed that 85% of teacher candidates are able to determine the relationship of science and local knowledge correctly. The ability to link the two domains, through the literature review, observation and interviews.
ARGUMENTS SKILLS ENHANCEMENT THROUGH THE IMPLEMENTATION OF PROBLEM BASED LEARNING IN 10th GRADE STUDENTS OF MIA 1 SMA BATIK 2 SURAKARTA Pritasari, A. C.; Dwiastuti, S.; Probosari, R. M.; Sajidan, S.
Jurnal Pendidikan IPA Indonesia Vol 4, No 2 (2015): October 2015
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v4i2.4185

Abstract

This research was aimed to improve the students’ argumentation skill in X MIA 1 SMA Batik 2 Surakarta Academic Year 2014/2015 through the implementation of problem based learning. This research is a classroom action research which consisted of three cycles. Each cycle contained planning, acting, observing and reflecting. The subjects of this research were students in X MIA 1 of SMA  Batik 2 Surakarta Academic Year 2014/2015. The data was collected using test, interview, and documentation. The data validation used triangulation technic. The data were analyzed descriptive was based on three components, they are: data reduction, data presentation and drawing the conclusion or verification. The research procedure is using spiral method. The results showed that argumentation skill of students increase from pre cycle, cycle I, cycle II, and cycle III. The average of students’ argumentation skill increase from 28,96% to 50,06%. Claim aspect increase from 61,62% to 82,49%; evidence aspect increase from 15,66% to 37,37%; and reasoning aspect increase from 9,60% to 30,30%. Based on the result of the research, the conclusion is there are improvement of argumentation skill through implementation of problem based learning in X MIA 1 SMA Batik 2 Surakarta.
Effectiveness of Critical Thinking Indicator-Based Module in Empowering Student’s Learning Outcome in Respiratory System Study Material Khasanah, A. N.; Widoretno, S.; Sajidan, S.
Jurnal Pendidikan IPA Indonesia Vol 6, No 1 (2017): April 2017
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v6i1.8490

Abstract

Research and development are aiming to develop critical thinking skills-based modules on the respiration system materials to empower the learning outcomes; verify the effectiveness of critical thinking skills-based modules with teaching materials in schools based on student learning outcomes. Research and development method are using Borg & Gall development procedure with nine phases. Data analysis that used for the research and development are qualitative and quantitative descriptive. Research and development results show that critical thinking skills-based modules on the respiration system materials were developed according to the indicators of critical thinking skills and knowledge dimension that visualised on the objectives, materials, activities and evaluation questions which are developed to empower the learning outcomes. The result of module effectiveness test, showed by Anacova test result are FCount = 180,4 on factual, conceptual, procedural learning outcomes which are visualised in the form of multiple choice questions and FCount = 40.2 metacognitive learning results are visualised in the form of essays.
PENGEMBANGAN MODUL BERBASIS INQUIRY LAB PADA MATERI SISTEM GERAK UNTUK MENINGKATKAN HASIL BELAJAR SISWA KELAS XI SMAN 1 MEJAYAN Pranoto, Andrias Marstanto Setyo; Sajidan, Sajidan; Prayitno, Baskoro Adi
DIDAKTIKA BIOLOGI: Jurnal Penelitian Pendidikan Biologi Vol 1, No 1 (2017): Maret 2017
Publisher : Universitas Muhammadiyah Palembang

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Abstract

Tujuan penelitian adalah: 1) mengembangkan modul berbasis Inquiry Lab, 2) menguji kelayakan modul berbasis Inquiry Lab, dan 3) menguji efektivitas modul berbasis Inquiry Lab untuk meningkatkan hasil belajar siswa pada materi sistem gerak di SMA Negeri 1 Mejayan. Pengembangan modul berbasis Inquiry Lab mengacu pada 9 langkah model Research and Development (R&D) dari Borg & Gall (1983) meliputi: 1) penelitian dan pengumpulan data, 2) perencanaan, 3) pengembangan produk, 4) uji coba produk awal, 5) revisi produk I, 6) uji coba lapangan, 7) revisi produk II, 8) uji coba lapangan operasional, 9) revisi produk akhir. Hasil penelitian menunjukkan: 1) modul biologi Inquiry Lab pada materi sistem gerak yang dikembangkan menggunakan sintak Inquiry Lab (observasi, manipulasi, generalisasi, verifikasi, dan aplikasi); 2) kelayakan modul berbasis Inquiry Lab sebagai berikut: a) uji validasi ahli materi 93,00%, b) validasi ahli pengembangan desain 82,90%, c) validasi ahli perangkat 95,70%, d) validator praktisi (guru) 94,00%, dan e) uji kelompok kecil 83,82%; dan 3) modul berbasis Inquiry Lab pada materi sistem gerak efektif dalam meningkatkan hasil belajar siswa (Sig.=0,000 < α=0,05). This research aimed: 1) to develop an inquiry-based laboratory module, 2) to test the feasibility of an inquiry-based laboratory module, and 3) to test the effectiveness of an inquiry-based laboratory module to improve students’ outcomes on motion system material at class XI of State Senior High School (SMAN) 1 of Mejayan. The development of an inquiry-based laboratory module referred to nine steps of Research and Development (R&D) model from Borg & Gall (1983) that included: 1) research and data collection, 2) planning, 3) product development, 4) initial product test, 5) product revision I, 6) field test, 7) product revision II, 8) operational field test, 9) final product revision. The research results showed: 1) the developed inquiry-based laboratory module on motion system material used the Inquiry Lab syntax (observation, manipulation, generalization, verification, and application), 2) the feasibility of an inquiry-based laboratory module as followed: a) the validation test of material expert at 93.00%, b) the validation of design development expert at 82.90%, c) the validation of tool expert at 95.70%, d) the validation of practitioners (teachers) at 94.00%, and e) the test of small group at 83.82%; and 3) an inquiry-based laboratory module on motion system material was effective to improve students’ outcomes (Sig.=0.000 < α=0.05).
PENGEMBANGAN MODUL BERBASIS INQUIRY LAB PADA MATERI SISTEM GERAK UNTUK MENINGKATKAN HASIL BELAJAR SISWA KELAS XI SMAN 1 MEJAYAN Pranoto, Andrias Marstanto Setyo; Sajidan, Sajidan; Prayitno, Baskoro Adi
DIDAKTIKA BIOLOGI: Jurnal Penelitian Pendidikan Biologi Vol 1, No 1 (2017): DIDAKTIKA BIOLOGI
Publisher : Universitas Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tujuan penelitian adalah: 1) mengembangkan modul berbasis Inquiry Lab, 2) menguji kelayakan modul berbasis Inquiry Lab, dan 3) menguji efektivitas modul berbasis Inquiry Lab untuk meningkatkan hasil belajar siswa pada materi sistem gerak di SMA Negeri 1 Mejayan. Pengembangan modul berbasis Inquiry Lab mengacu pada 9 langkah model Research and Development (R&D) dari Borg & Gall (1983) meliputi: 1) penelitian dan pengumpulan data, 2) perencanaan, 3) pengembangan produk, 4) uji coba produk awal, 5) revisi produk I, 6) uji coba lapangan, 7) revisi produk II, 8) uji coba lapangan operasional, 9) revisi produk akhir. Hasil penelitian menunjukkan: 1) modul biologi Inquiry Lab pada materi sistem gerak yang dikembangkan menggunakan sintak Inquiry Lab (observasi, manipulasi, generalisasi, verifikasi, dan aplikasi); 2) kelayakan modul berbasis Inquiry Lab sebagai berikut: a) uji validasi ahli materi 93,00%, b) validasi ahli pengembangan desain 82,90%, c) validasi ahli perangkat 95,70%, d) validator praktisi (guru) 94,00%, dan e) uji kelompok kecil 83,82%; dan 3) modul berbasis Inquiry Lab pada materi sistem gerak efektif dalam meningkatkan hasil belajar siswa (Sig.=0,000 < a=0,05). This research aimed: 1) to develop an inquiry-based laboratory module, 2) to test the feasibility of an inquiry-based laboratory module, and 3) to test the effectiveness of an inquiry-based laboratory module to improve students’ outcomes on motion system material at class XI of State Senior High School (SMAN) 1 of Mejayan. The development of an inquiry-based laboratory module referred to nine steps of Research and Development (R&D) model from Borg & Gall (1983) that included: 1) research and data collection, 2) planning, 3) product development, 4) initial product test, 5) product revision I, 6) field test, 7) product revision II, 8) operational field test, 9) final product revision. The research results showed: 1) the developed inquiry-based laboratory module on motion system material used the Inquiry Lab syntax (observation, manipulation, generalization, verification, and application), 2) the feasibility of an inquiry-based laboratory module as followed: a) the validation test of material expert at 93.00%, b) the validation of design development expert at 82.90%, c) the validation of tool expert at 95.70%, d) the validation of practitioners (teachers) at 94.00%, and e) the test of small group at 83.82%; and 3) an inquiry-based laboratory module on motion system material was effective to improve students’ outcomes (Sig.=0.000 < a=0.05).
Science Integrated Learning Model to Enhance The Scientific Work Independence of Student Teacher in Indigenous Knowledge Transformation Parmin, P.; Sajidan, S.; Ashadi, A.; Sutikno, S.; Fibriana, F.
Jurnal Pendidikan IPA Indonesia Vol 6, No 2 (2017): October 2017
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v6i2.11276

Abstract

The scientific work independence is the core competency of student teacher of science. In this research, the effectiveness of the Science Integrated Learning Model (SIL) was measured in term of the scientific work independence of student teacher of science in changing the society’s original knowledge into scientific knowledge. The changing was measured through Ethnoscience learning. The experimental method was used with the control group and experimental group in three different universities. The result of the t-test shows a correlation coefficient significance value at 0.000 < 0.05. Therefore, it concludes that there is not any difference between the experimental and control group. However, there is an effect of model application on the independence of the scientific work of student teacher of science.  
SKILL OF TEACHER CANDIDATES IN INTEGRATING THE CONCEPT OF SCIENCE WITH LOCAL WISDOM Parmin, P.; Sajidan, S.; Ashadi, A.; Sutikno, S.
Jurnal Pendidikan IPA Indonesia Vol 4, No 2 (2015): October 2015
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v4i2.4179

Abstract

Learning science is not limited to reviewing the concepts, but strengthens the identity of a nation that has a diversity of cultures. Science learning objectives that have been set in Indonesia, including the student is able to apply the science wisely, to maintain and preserve the cultural survival. The study aims to measure students' ability to relate concepts of science with local knowledge to use mind maps compiled individually. The results showed that 85% of teacher candidates are able to determine the relationship of science and local knowledge correctly. The ability to link the two domains, through the literature review, observation and interviews.
ARGUMENTS SKILLS ENHANCEMENT THROUGH THE IMPLEMENTATION OF PROBLEM BASED LEARNING IN 10th GRADE STUDENTS OF MIA 1 SMA BATIK 2 SURAKARTA Pritasari, A. C.; Dwiastuti, S.; Probosari, R. M.; Sajidan, S.
Jurnal Pendidikan IPA Indonesia Vol 4, No 2 (2015): October 2015
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v4i2.4185

Abstract

This research was aimed to improve the students’ argumentation skill in X MIA 1 SMA Batik 2 Surakarta Academic Year 2014/2015 through the implementation of problem based learning. This research is a classroom action research which consisted of three cycles. Each cycle contained planning, acting, observing and reflecting. The subjects of this research were students in X MIA 1 of SMA  Batik 2 Surakarta Academic Year 2014/2015. The data was collected using test, interview, and documentation. The data validation used triangulation technic. The data were analyzed descriptive was based on three components, they are: data reduction, data presentation and drawing the conclusion or verification. The research procedure is using spiral method. The results showed that argumentation skill of students increase from pre cycle, cycle I, cycle II, and cycle III. The average of students’ argumentation skill increase from 28,96% to 50,06%. Claim aspect increase from 61,62% to 82,49%; evidence aspect increase from 15,66% to 37,37%; and reasoning aspect increase from 9,60% to 30,30%. Based on the result of the research, the conclusion is there are improvement of argumentation skill through implementation of problem based learning in X MIA 1 SMA Batik 2 Surakarta.
Co-Authors - Fakhrurrazi A. Ashadi, A. A. C. Pritasari, A. C. A. N. Khasanah, A. N. A. Wibowo A.A. Ketut Agung Cahyawan W A.M.P. Nuhriawangsa Achrudin Achrudin Adelia Anisa Adi Magna Patriadi Nuhriawangsa Adi Ratriyanto Adi, Fadhil Purnama Afandi Afandi Agung Budiharjo Agus Efendi Ahmad Khoiri Ahmad Makmur Santoso Alisah, Siti Ana Susanti Andriani, Lutfi Andrias Marstanto Setyo Pranoto, Andrias Marstanto Setyo Anggit Sasmito, Anggit Anisyah Yuniarti Anjarwati, Pamula Guruh Prastiwa ARI SUSILOWATI Arief B. Witarto Ariyanto J ARTINI PANGASTUTI Ashadi Ashari, Dhita Karunia Asnafiyah, Asnafiyah Atika GA, Atika ATIKA GURITNA AYU, ATIKA GURITNA Atmojo, I. R. W. Ayu Rahma Ulufa Nuri Aziza Karenina, Aziza Baskoro Adi Prayitno Baskoro Adi Prayitno Baskoro Adi Prayitno Baskoro Baskoro, Baskoro Basori Basori Bowo Sugiharto Catur Suci Purwati Chrisnia Octovi Chumdari Chumdari, Chumdari Deny Febriana, Deny Desy Fajar Priyayi Devita Yudhayanti, Devita Dewanto Harjunowibowo Dewi - Rochsantiningsih Dewi Anjani, Dewi Dewi Puspita Sari Dhora Vasminingtya R.C, Dhora Dian Kurvayanti Innatesari Dito, Bahana Safiria Drajat Tri Kartono Dwi Hartanti Dwi Hartanti DWI OETOMO Dwi Oetomo Dwi Retno Sari, Dwi Retno Dwi Setyo Astuti Effendi, Bob Soelaiman Eko Setyaningsih, Eko Elok Norma Khabibah, Elok Norma Endang Purwanti Ervan Johan Wicaksono, Ervan Johan Etviana, R. Evi Elisanti Evi Elisanti, Evi Evi Noor Hidayah Fadhil Purnama Adi Fatih Ngaisah Wijayanti, Fatih Ngaisah Febri, Ade Fibriana, F. Fitriana Dwi Utari, Fitriana Dwi Fitriyana Rachmah Gilang Mahendrati Greiner, Ralf Hailu Weldekiros Hailu, Hailu Weldekiros Hanni Hanifah Harlita Harlita Hastjarjo, Sri Heni Pujiastuti Hephi Meilinda, Hephi Herning Nurdiana Hidayatullah, Muhammad Eka Himawan, Widhi Hudin, Robertus I Gusti Ngurah Antaryama I Nyoman Tri Bayu Tanaya, I Nyoman Idam Ragil Widianto Atmojo Idam Ragil Widianto Atmojo Idhun Prasetyo Riyadi, Idhun Prasetyo Innatesari, Dian Kurvayanti Irma Yuniar Wardhani Isma Aziz Fakhrudin Jayawarsa, A.A. Ketut Joko Ariyanto Joko Sulianto . . Konitah, Zahra Latif Agung Nugroho, Latif Agung Lilia Halim, Lilia M. Akhyar, M. M. Noris Mahfudhoh, Nimatul Laili Nur Maridi - - Maridi Maridi Maridi Maridi Maridi, M Marinda Mega Nurfitriani, Marinda Mega Meti Indrowati Misbahul Jannah Mkumbe, Baraka Stewart Moh Salimi Mohammad Masykuri Mufida Nofiana Muhammad Akhyar Munawwaroh Kurniawati, Munawwaroh Murni Ramli Murni, Odela Priscilia Mustikasari, Intan Muzzazinah Muzzazinah N. SURYANI Nana - - Nancy, Natasha Nanik Murti Prasetyanti Nanik Murti Prasetyanti, Nanik Murti Nulngafan, Nulngafan Nunuk Suryani Nur Khasanah Nur Khasanah Nurdiana, Herning Nuri, Ayu Rahma Ulfa Nuri, Ayu Rahma Ulufa Nurmiyati Nurmiyati Pamula Guruh, Pamula Parmin - Parmin Parmin Permatasari, Nila Prabang Setyono Pramesti, Betty Novia Prayitno, B. A. Puguh - Karyanto PUGUH KARYANTO Puput Dwi Maret Tanti R. M. Probosari Rahmawati Ika Listyaningrum Riezky Maya Probosari Rifkie Aziz Agustian, Rifkie Aziz Rini Setyowati, Rini Riyadi Riyadi Rizki Agung, Rizki Rizki Permata Yusniawati, Rizki Permata Roslan, Roslinawati Mohd Rosmala Setiawati, Rosmala Roy Ardiansyah Roy Ardiansyah, Roy S Suciati S. Dwiastuti, S. S. Sutarno, S. S. Widoretno, S. Sabila, Wahdania Nuris Santoso S Saputri, D. Y. Saputri, Dwi Yuniasih Saraswati, Zahra Nadhila Sarwanto Sarwanto Septina Dwi Prasetyana, Septina Dwi Setiani, Indra Setria Utama Rizal Shinta Devi Amielia, Shinta Devi Sindy Nurinda, Sindy Singgih Prihadi Siswandari Siswandari siti wulandari Slamet Santosa Sri Dwiastuti Sri Widoretno Suciati Suciati Suciati Sudarisman Sugiyanto Sugiyanto Sugiyarto Sugiyarto Sukarmin Sulistyawan, Herwanto Gede Sulistyo Saputro Sunarto Sunarto Suranto Suranto Suranto Suranto Suranto, S. Suraya, Ana Susilowati Susilowati Sutarno Sutarno Sutarno Sutarno Sutikno Madnasri Suwarno Suwarno Suyitno, Margiyono Tarliany, Eny Tina Nur Khasanah, Tina Nur Tri - Novana Twiningsih, Anik UMI FATMAWATI Ummi Nur Afinni Dwi Jayanti, Ummi Nur Afinni Utami, Fitri Astuti Wahyu Walid, Ahmad Widha Sunarno Wijarini, Fitri - Wijayanti, Sovia Wikara, B. Winarno, Widha Winarsih - Wiranto Wiranto Wulandari Wulandari Yanti, Amalia Firdaus Yasinta Choirina Yasir Sidiq Yenny Putri Pratiwi, Yenny Putri Yuhana Yuhana, Yuhana Yuliati - Z. Bachruddin